MYH6 通过下调人前列腺癌细胞中 KIT 的表达来抑制肿瘤进展。

MYH6 suppresses tumor progression by downregulating KIT expression in human prostate cancer.

机构信息

Department of Urology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, 26 Daoqian Rd, Suzhou, 215000, Jiangsu, People's Republic of China.

出版信息

Sci Rep. 2024 Aug 24;14(1):19685. doi: 10.1038/s41598-024-70665-3.

DOI:10.1038/s41598-024-70665-3

PMID:39181964

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11344859/

Abstract

Prostate cancer (PRAD) is one of the leading malignancies in men all around the world. Here, we identified Myosin Heavy Chain 6 (MYH6) as a potential tumor suppressor gene in the development of prostate cancer. We found lower expression of MYH6 in prostate cancer tissues, and its lower gene expression was also associated with worse clinical outcomes. In vitro and in vivo assays indicated that overexpressed MYH6 could suppress the proliferation and migration progression of prostate cancer cells. RNA-seq was employed to investigate the mechanism, and KIT Proto-Oncogen (KIT) was determined as the downstream gene of MYH6, which was further confirmed using rescue assays. In all, we provide the evidence that MYH6 could serve as a tumor suppressor in prostate cancer. Our results highlight the potential role of MYH6 in the development of prostate cancer.

摘要

前列腺癌（PRAD）是全球男性中主要的恶性肿瘤之一。在这里，我们鉴定肌球蛋白重链 6（MYH6）作为前列腺癌发展中的一个潜在的肿瘤抑制基因。我们发现 MYH6 在前列腺癌组织中的表达降低，其较低的基因表达也与更差的临床结局相关。体外和体内实验表明，过表达 MYH6 可以抑制前列腺癌细胞的增殖和迁移进展。RNA-seq 被用来研究机制，KIT 原癌基因（KIT）被确定为 MYH6 的下游基因，这一结论通过挽救实验进一步得到证实。总之，我们提供了 MYH6 可以作为前列腺癌的肿瘤抑制因子的证据。我们的研究结果强调了 MYH6 在前列腺癌发展中的潜在作用。