Department of Cytology, Institute for Biological Research "Siniša Stanković" - National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd., 142, Belgrade 11108, Serbia.
Department of Cytology, Institute for Biological Research "Siniša Stanković" - National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blvd., 142, Belgrade 11108, Serbia.
Ann Anat. 2024 Oct;256:152322. doi: 10.1016/j.aanat.2024.152322. Epub 2024 Aug 23.
Vitamin D supplementation in aging subjects manifests a positive effect on various health-related parameters. We performed a functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output after vitamin D3 application in a rat model of the andropause.
Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3-treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.
Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.
Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.
维生素 D 补充剂在衰老受试者中表现出对各种与健康相关参数的积极影响。我们对去势雄鼠模型的肾上腺皮质进行了功能组织学分析，研究了维生素 D 活性和皮质酮输出的因素。
中年 Wistar 大鼠分为假手术（SO；n=8）、去势（Orx；n=8）和维生素 D3 处理去势（Orx+vit. D；n=8）组。维生素 D3（5μg/kg b.m.）皮下给药 3 周，而 SO 和 Orx 组仅给予载体。通过组织化学/免疫组织化学、体视学、超微结构和生化分析来实现设定的目标。
去势（Orx）降低了肾上腺皮质相关的血管（p<0,0001）、维生素 D 受体（VDR；p<0,0166）、细胞色素 P450 氧化酶 2R1（CYP 2R1；p<0,0001）和细胞色素 P450 氧化酶 24（CYP 24；p<0,0001）库的体积密度，但增加了细胞色素 P450 27B1（CYP 27B1；p<0,0001）库的体积密度。在 Orx+vit. D 大鼠中，观察到肾上腺皮质相关胶原（p<0,0001）、VDR（p<0,0001）和 CYP 2R1（p<0,0001）库以及肾上腺皮质外束状带细胞中脂滴直径（p<0,0001）的体积密度增加，而血管（p<0,0001）、CYP 27B1（p<0,0001）和 CYP 24（p<0,0001）库的体积密度降低，所有这些都与 Orx 组相比。ACTH 血浆水平降低（p=0,0155），25-羟维生素 D3 和皮质酮的血清浓度升高（p<0,0001 和 p=0,0187，分别），所有这些都是在相同的治疗后发生的。
去势雄鼠应用维生素 D3 后皮质酮输出增加似乎与肾上腺组织中 25-羟维生素 D3 可用性增加和 1,25-二羟维生素 D3 降解减少无关，而是涉及中枢调节机制。
