Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
J Agric Food Chem. 2024 Sep 4;72(35):19333-19341. doi: 10.1021/acs.jafc.4c06062. Epub 2024 Aug 25.
The regulation solutions and mechanisms of reducing pesticide phytotoxicity to nontarget plants are not well-defined and detailed. Here, we have proposed a new detoxification strategy to control the toxic effects of herbicide imazethapyr (IM) induced in wheat seedlings from the perspective of the plasma membrane (PM) H-ATPase. We found that the changes in PM H-ATPase activity have a regulatory effect on the phytotoxic effects induced by IM in plants. Treatment with PM H-ATPase activators restored the reduced auxin content and photosynthetic efficiency caused by IM, thereby promoting plant growth. Application of a PM H-ATPase inhibitor further reduced phosphorus content and significantly increased 2,4-dihydroxy-7-methoxy-2,1,4-benzoxazin-3(4)one (DIMBOA) and jasmonic acid levels. These effects indicate that auxin and DIMBOA may regulate plant growth trends and detoxification effects mediated by PM H-ATPase. This work opens a new strategy for regulating herbicide toxicity to nontarget plants from the PM H-ATPase.
降低农药对非靶标植物的植物毒性的调控解决方案和机制尚不清楚和详细。在这里，我们从质膜（PM）H+-ATPase 的角度出发，提出了一种新的解毒策略来控制除草剂咪唑乙烟酸（IM）诱导的小麦幼苗的毒性作用。我们发现，PM H+-ATPase 活性的变化对 IM 诱导的植物毒性有调节作用。PM H+-ATPase 激活剂的处理恢复了 IM 引起的生长素含量和光合作用效率的降低，从而促进了植物的生长。PM H+-ATPase 抑制剂的应用进一步降低了磷含量，显著增加了 2,4-二羟基-7-甲氧基-2,1,4-苯并恶嗪-3(4)-酮（DIMBOA）和茉莉酸的水平。这些作用表明，生长素和 DIMBOA 可能调节由 PM H+-ATPase 介导的植物生长趋势和解毒作用。这项工作为从 PM H+-ATPase 调节除草剂对非靶标植物的毒性开辟了一条新的策略。
