Apel R, Delling G, Krumme H, Winkler K, Salzer-Kuntschik M
Virchows Arch A Pathol Anat Histopathol. 1985;405(2):215-23. doi: 10.1007/BF00704373.
A current strategy for osteosarcoma treatment is neo-adjuvant chemotherapy prior to the resection of the tumour. It appears that some tumours respond very well to the cytostatic therapy, while others show little or no effect. It is desirable to be able predict the response of the tumour before starting chemotherapy. 16 biopsy specimens from patients with osteosarcoma who had been treated according to the protocol of the study COSS-80 and COSS-82 were examined. 100 tumour cells from each biopsy have been measured by an electronic interactive image analysis system (IBAS II; Kontron/ZEISS). After completion of chemotherapy en bloc resection of the tumour was performed. The entire surgical specimen was completely examined at two levels by means of undecalcified sections, and assigned a grade for the effect of chemotherapy analogous to the grading of Salzer-Kuntschik et al. (1983). The quantitative analysis of tumour cell nuclei revealed two different patterns of nuclear sizes, which were correlated significantly with the chemotherapy response (P less than 0.002). Tumour cell nuclei of well responders were significantly larger and showed a greater variance in size (mean value 66 + 41 micron 2), than those of poor responders (mean value 38 + 18 micron 2). We conclude from our results that quantitative analysis and classification of nuclear size of osteosarcoma cells may be useful for predicting chemotherapy response in patients with osteosarcoma.
骨肉瘤治疗的当前策略是在肿瘤切除前进行新辅助化疗。似乎有些肿瘤对细胞抑制疗法反应良好，而另一些则几乎没有效果或完全无效。在开始化疗之前能够预测肿瘤的反应是很有必要的。对16例按照COSS - 80和COSS - 82研究方案接受治疗的骨肉瘤患者的活检标本进行了检查。通过电子交互式图像分析系统（IBAS II；康强/蔡司）对每份活检标本中的100个肿瘤细胞进行了测量。化疗完成后，对肿瘤进行整块切除。通过不脱钙切片在两个层面上对整个手术标本进行全面检查，并根据类似于Salzer - Kuntschik等人（1983年）的分级方法为化疗效果评定等级。肿瘤细胞核的定量分析揭示了两种不同的核大小模式，它们与化疗反应显著相关（P小于0.002）。化疗反应良好者的肿瘤细胞核明显更大，大小差异也更大（平均值为66 + 41微米²），而反应较差者的肿瘤细胞核（平均值为38 + 18微米²）则不然。我们从结果中得出结论，骨肉瘤细胞核大小的定量分析和分类可能有助于预测骨肉瘤患者的化疗反应。
