Stenkvist B, Westman-Naeser S, Holmquist J, Nordin B, Bengtsson E, Vegelius J, Eriksson O, Fox C H
Cancer Res. 1978 Dec;38(12):4688-97.
A new method for measuring differences in nuclear detail in chrome alum gallocyanin-stained nuclei of cells from human breast cancers was compared with conventional subjective grading and classification systems. The new method, termed computerized nuclear morphometry (CNM), gives a multivariate numerical score that correlates well with nuclear atypia and gives a higher reproducibility of classification than do subjective observations with conventional histological preparations. When 100 individual nuclei from each of 137 breast cancers were examined by CNM, there was a broad CNM score variation between patients but a good reproducibility for each tumor. When different parts of the same tumor were sampled, there was good reproducibility between samples, indicating that some breast cancers at least are "geometrically monoclonal." When these cancers were compared by the grading systems of WHO and Black, correlations of 0.43 and 0.48, respectively, were found. There was a poor correlation between CNM and classifications of tumor type, but in general there were high values for CNM in medullary tumors and low values in mucous tumors. Correlations between CNM and tumor progression and prognosis await future study of patients participating in the study.
一种用于测量人类乳腺癌细胞经铬明矾龙胆紫染色的细胞核中核细节差异的新方法，与传统的主观分级和分类系统进行了比较。这种新方法被称为计算机化核形态测量法（CNM），它给出了一个多变量数值评分，该评分与核异型性密切相关，并且与使用传统组织学标本的主观观察相比，具有更高的分类可重复性。当通过CNM检查137例乳腺癌中每例的100个单个细胞核时，患者之间的CNM评分存在广泛差异，但每个肿瘤的可重复性良好。当对同一肿瘤的不同部位进行采样时，样本之间具有良好的可重复性，这表明至少某些乳腺癌是“几何单克隆的”。当根据世界卫生组织（WHO）和布莱克（Black）的分级系统对这些癌症进行比较时，发现相关性分别为0.43和0.48。CNM与肿瘤类型分类之间的相关性较差，但一般来说，髓样肿瘤的CNM值较高，黏液性肿瘤的CNM值较低。CNM与肿瘤进展和预后之间的相关性有待参与该研究的患者的未来研究。
