Histomorphometry of spherical tumors using holoptical cross-sections.

A new morphometric method for quantifying tumour regression on holoptical cross-sections is described which permits assessment of the extent and distribution of viable tumour tissue and necrosis in spherical tumours. The value of the method is demonstrated by comparing tumour regression in the liver metastases of a colorectal adenocarcinoma before and after regional chemotherapy. The practicability of the method for clinicopathological therapeutic studies is emphasized.