体外评估[具体提取物名称]提取物的细胞保护和基因保护作用。 （你提供的原文中“and”前似乎少了提取物名称相关内容）
Assessment of cytoprotective and genoprotective effects of and extracts in vitro.
作者信息
Bagri Preeti, Kumar Vinod, Batra Kanisht
机构信息
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India.
Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India.
出版信息
Toxicol Res (Camb). 2024 Aug 22;13(4):tfae133. doi: 10.1093/toxres/tfae133. eCollection 2024 Aug.
BACKGROUND
and is extensively used as an ingredient of food and in traditional medicine for the management of a variety of diseases.
MATERIAL AND METHODS
The extracts of leaf of and stem of were assessed to examine their ability to inhibit the oxidative DNA damage (by DNA protection assay), cytoprotective and genoprotective potential (by Comet assay) in V79 cells individually and in combinations.
RESULT
It was found that these extracts could significantly inhibit the OH-dependent damage of pUC18 plasmid DNA. extract (160 and 320 μg/mL) and extract (640, 1,280 and 2,560 μg/mL) individually showed higher DNA protection activity. (1,280 μg/mL) combined with (640 μg/mL) showed best cytoprotective and genoprotective activities among different concentration combinations and various concentrations of individual plants in V79 cell line against hydrogen peroxide induced cytotoxicity and genotoxicity.
CONCLUSION
This study demonstrates the cytoprotective and genoprotective activity of M. oleifera and Tinospora cordifolia individually or in combination.
背景
某某物质被广泛用作食品成分以及传统医学中用于治疗多种疾病。
材料与方法
对某某植物的叶提取物和某某植物的茎提取物进行评估，以单独及组合方式检测它们在V79细胞中抑制氧化DNA损伤（通过DNA保护试验）、细胞保护和基因保护潜力（通过彗星试验）的能力。
结果
发现这些提取物可显著抑制pUC18质粒DNA的羟基依赖性损伤。某某植物提取物（160和320μg/mL）和某某植物提取物（640、1280和2560μg/mL）单独显示出较高的DNA保护活性。在V79细胞系中，某某植物提取物（1280μg/mL）与某某植物提取物（640μg/mL）组合在不同浓度组合以及各植物不同浓度下，针对过氧化氢诱导的细胞毒性和基因毒性显示出最佳的细胞保护和基因保护活性。
结论
本研究证明了辣木和心叶马兜铃单独或联合使用时的细胞保护和基因保护活性。
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