Chatterjee Dipanjali, Mitra Adinpunya
Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721302 India.
Physiol Mol Biol Plants. 2024 Aug;30(8):1363-1381. doi: 10.1007/s12298-024-01489-8. Epub 2024 Jul 19.
Mints are aromatic plants of , globally known for the phytochemical-rich essential oils. Most of the cultivated mints are menthol-rich, whereas spearmint being the only dominant carvone-rich species. In this study, another carvone-rich mint (L.) Huds., a native of temperate region was assessed for its acclimation in sub-tropical environment to see any possible changes in specialized metabolite accumulation. Plants grown under open environment was compared with glasshouse grown plants where, temperature, humidity and photoperiods were uniformly maintained. Thickened leaves with increased cuticular wax load (2.82 folds) and anthocyanin accumulation (202.97 µg/g) in the widened stems were observed in plants grown in open environment, while higher chlorophyll contents were exhibited by the glasshouse-grown plants. Enhanced antioxidant capacity in open environment, correlated with elevated concentration (86.4% increase for caffeic acid) of wall-bound phenolics was observed. Increased proline, hydrogen peroxide and malondialdehyde contents in open environment indicated the plant's ability to cope up with abiotic stress. Higher amounts of terpenes like (-)-carvone (2.68 folds) and D-limonene (1.35 folds) were found in both internal volatile pool and essential oil of glasshouse-grown plants. Histochemical study of glandular trichomes also supported this finding. In conclusion, glasshouse-grown plants showed relatively better growth and higher terpene contents, nevertheless the plant survived well in warmer environment, with increased antioxidant capacities and phenolic contents. Future study includes mass propagation of this species in different geographical locations with distinct climatic variations to determine the suitable sub-tropical locations for cultivation as a potential alternative to spearmint for commercial-scale (-)-carvone production.
The online version contains supplementary material available at 10.1007/s12298-024-01489-8.
薄荷是唇形科的芳香植物，全球闻名于富含植物化学物质的精油。大多数栽培薄荷富含薄荷醇，而留兰香是唯一主要富含香芹酮的品种。在本研究中，对另一种原产于温带地区、富含香芹酮的薄荷（水薄荷）在亚热带环境中的适应性进行了评估，以观察其特殊代谢产物积累的任何可能变化。将在开放环境中生长的植物与在温室中生长的植物进行比较，温室中温度、湿度和光周期保持一致。在开放环境中生长的植物，叶片变厚，角质蜡负载增加（2.82倍），茎变宽处花青素积累（202.97μg/g），而温室种植的植物叶绿素含量更高。在开放环境中观察到抗氧化能力增强，与细胞壁结合酚类物质浓度升高（咖啡酸增加86.4%）相关。开放环境中脯氨酸、过氧化氢和丙二醛含量增加表明植物应对非生物胁迫的能力。在温室种植植物的内部挥发物库和精油中都发现了更高含量的萜类化合物，如（-）-香芹酮（2.68倍）和D-柠檬烯（1.35倍）。腺毛的组织化学研究也支持了这一发现。总之，温室种植的植物生长相对较好，萜类化合物含量较高，尽管如此，该植物在温暖环境中存活良好，抗氧化能力和酚类物质含量增加。未来的研究包括在不同气候差异明显的地理位置对该物种进行大规模繁殖，以确定适合作为留兰香商业规模生产（-）-香芹酮潜在替代品的亚热带种植地点。
在线版本包含可在10.1007/s12298-024-01489-8获取的补充材料。
