Jan Rahmatullah, Asif Saleem, Asaf Sajjad, Khan Zakirullah, Kim Kyung-Min
Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu, Republic of Korea.
Coastal Agriculture Research Institute, Kyungpook National University, Daegu, Republic of Korea.
Front Plant Sci. 2024 May 28;15:1397817. doi: 10.3389/fpls.2024.1397817. eCollection 2024.
This study investigates the impact of anthocyanin treatment on rice plants under drought stress, focusing on phenotypic, molecular, and biochemical responses. Anthocyanin were treated to one month old plants one week before the droughtexposure. Drought stress was imposed by using 10% polyethylene glycol (PEG 6000). Anthocyanin-treated plants exhibited significant enhancements in various traits, including growth parameters and reproductive characteristics, under normal conditions. When subjected to drought stress, these plants displayed resilience, maintaining or improving essential morphological and physiological features compared to non-treated counterparts. Notably, anthocyanin application mitigated drought-induced oxidative stress, as evidenced by reduced levels of reactive oxygen species (ROS) and lipid membrane peroxidation. The study also elucidates the regulatory role of anthocyanins in the expression of flavonoid biosynthetic genes, leading to increased levels of key secondary metabolites. Furthermore, anthocyanin treatment influenced the levels of stress-related signaling molecules, including melatonin, proline, abscisic acid (ABA), and salicylic acid (SA), contributing to enhanced stress tolerance. The enzymatic activity of antioxidants and the expression of drought-responsive genes were modulated by anthocyanins, emphasizing their role in antioxidant defense and stress response. Additionally, anthocyanin treatment positively influenced macronutrient concentrations, particularly calcium ion (Ca), potassium ion (K), and sodium ion (Na), essential for cell wall and membrane stability. The findings collectively highlight the multifaceted protective effects of anthocyanins, positioning them as potential key players in conferring resilience to drought stress in rice plants. The study provides valuable insights into the molecular and physiological mechanisms underlying anthocyanin-mediated enhancement of drought stress tolerance, suggesting promising applications in agricultural practices for sustainable crop production.
本研究调查了花青素处理对干旱胁迫下水稻植株的影响，重点关注其表型、分子和生化反应。在干旱处理前一周，对一个月龄的植株进行花青素处理。使用10%聚乙二醇（PEG 6000）施加干旱胁迫。在正常条件下，经花青素处理的植株在各种性状上表现出显著增强，包括生长参数和生殖特性。在遭受干旱胁迫时，这些植株表现出恢复力，与未处理的对照相比，维持或改善了重要的形态和生理特征。值得注意的是，花青素的施用减轻了干旱诱导的氧化应激，活性氧（ROS）水平和脂质膜过氧化程度降低证明了这一点。该研究还阐明了花青素在类黄酮生物合成基因表达中的调控作用，导致关键次生代谢产物水平增加。此外，花青素处理影响了与胁迫相关的信号分子水平，包括褪黑素、脯氨酸、脱落酸（ABA）和水杨酸（SA），有助于增强胁迫耐受性。花青素调节了抗氧化剂的酶活性和干旱响应基因的表达，强调了它们在抗氧化防御和胁迫反应中的作用。此外，花青素处理对大量营养素浓度产生了积极影响，特别是对细胞壁和膜稳定性至关重要的钙离子（Ca）、钾离子（K）和钠离子（Na）。这些发现共同突出了花青素的多方面保护作用，使其成为赋予水稻植株抗旱胁迫恢复力的潜在关键因素。该研究为花青素介导增强干旱胁迫耐受性的分子和生理机制提供了有价值的见解，为可持续作物生产的农业实践提出了有前景的应用。
