Andrade Andrea, Escalante Maximiliano, Ramírez Federico, Vigliocco Ana, Alemano Sergio
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, UNRC, Instituto de Investigaciones Agrobiotecnológicas-Consejo Nacional de Investigaciones Científicas y Técnicas (INIAB-CONICET), 5800 Río Cuarto, Argentina.
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas, y Naturales, Universidad Nacional de Rio Cuarto (UNRC), 5800 Río Cuarto, Argentina.
Physiol Mol Biol Plants. 2024 Aug;30(8):1277-1295. doi: 10.1007/s12298-024-01497-8. Epub 2024 Aug 5.
Water deficit stress reduces crop yield in field crops, including sunflowers, at any growth stage. In response, most plants activate hormonal and gene expression patterns to mitigate damage. In this study, we evaluated changes in the physiological and gene transcription levels of two sunflower ( L.) inbred lines -one sensitive (B59 line) and one water stress-tolerant (B71)-in response to water stress, by using mannitol to simulate water deficit conditions, which provides moderate stress in both sunflower lines. The analyses of the accumulation of various phytohormones under this stress revealed that Jasmonic acid (JA) significantly increased in the shoots of both lines. Similarly, Salicylic acid (SA) increased in the shoots of both lines, although it also accumulated in B71 roots. In addition, Abscisic acid (ABA) and Indole-3-acetic acid (IAA) showed a considerable increase in the B59 shoots. Regarding the JA and SA pathways, the WRKY70 transcription levels were higher in the shoots of both lines and the roots of B71. The B59 line showed overtranscription of a gene related to the ABA pathway (XERICO) and genes associated with IAA (ARF9 and ARF16 genes). The B71 line, on the other hand, simultaneously triggered the JA, SA and ABA hormonal pathways in response to this stress condition. The ABA and JA hormonal pathways activated different TFs, such as RD20, RD22, RD26, ANAC19 and ANAC29, through MYC2. Both the JA and SA hormonal pathways activated the WRKY70 transcription factor. Altogether, each line triggered the hormonal and transcriptional pathways in response to water stress, although at varying intensities. The results suggest that the hormonal pathways of JA, SA, IAA and ABA, along with their primary associated genes, are activated in response to water deficit at the early growth stage in sunflower seedlings, which mitigates damage.
The online version contains supplementary material available at 10.1007/s12298-024-01497-8.
水分亏缺胁迫会降低包括向日葵在内的大田作物在任何生长阶段的产量。作为响应，大多数植物会激活激素和基因表达模式以减轻损害。在本研究中，我们通过使用甘露醇模拟水分亏缺条件来评估两个向日葵（L.）自交系——一个敏感系（B59系）和一个耐水分胁迫系（B71）——在水分胁迫下生理和基因转录水平的变化，这种模拟为两个向日葵系都提供了适度胁迫。对这种胁迫下各种植物激素积累的分析表明，茉莉酸（JA）在两个系的地上部分均显著增加。同样，水杨酸（SA）在两个系的地上部分也增加，尽管它也在B71系的根中积累。此外，脱落酸（ABA）和吲哚 - 3 - 乙酸（IAA）在B59系的地上部分有相当大的增加。关于JA和SA途径，WRKY70转录水平在两个系的地上部分以及B71系的根中较高。B59系显示出与ABA途径相关的一个基因（XERICO）以及与IAA相关的基因（ARF9和ARF16基因）的过度转录。另一方面，B71系在这种胁迫条件下同时触发了JA、SA和ABA激素途径。ABA和JA激素途径通过MYC2激活了不同的转录因子，如RD20、RD22、RD26、ANAC19和ANAC29。JA和SA激素途径都激活了WRKY70转录因子。总之，每个系都响应水分胁迫触发了激素和转录途径，尽管强度不同。结果表明，JA、SA、IAA和ABA的激素途径及其主要相关基因在向日葵幼苗早期生长阶段响应水分亏缺时被激活，从而减轻损害。
在线版本包含可在10.1007/s12298 - 024 - 01497 - 8获取的补充材料。
