Liu Changfeng, Wu Huaixin, Wang Ye, Song Xinmei, Jia Chunyun
College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang, China.
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
Int J Phytoremediation. 2025 Jul 11:1-10. doi: 10.1080/15226514.2025.2527936.
Soil contamination by heavy metals is increasingly severe, particularly in eastern Inner Mongolia, China, where chromite mining has resulted in significant environmental issues due to heavy metal residues. Alfalfa, a common forage crop in the region, is considered an effective crop for remediating heavy metal contamination. However, the effectiveness of alfalfa alone in this context is limited. Jasmonic acid (JA), as a major phytohormone, plays an important role in regulating various physiological processes in plants; therefore, in this study, JA was added to remediate chromium contamination synergistically with alfalfa. The objective of this study was to investigate the synergistic effects of JA in combination with alfalfa for the remediation of Cr contamination. Specifically, the functional role of JA in modulating alfalfa growth and physiological responses under different Cr concentrations (1, 10, and 30 mg/L) was elucidated. JA application significantly reduced Cr accumulation in alfalfa seedlings by 49.70-87.37%, with mitigation efficacy dependent on Cr stress intensity: at 1 mg/L Cr, (0.1-1 μM) JA reduced the inhibitory effects on roots and stems by 74.86% and 270.91%, respectively. At 10 mg/L Cr, (0.5-1 μM) JA inhibited oxidative damage and reduced the levels of HO and O by 61.19% and 14.91%, respectively. For 30 mg/L Cr, 0.5 μM JA maximized total chlorophyll synthesis (2.03-fold increase) and root cell viability (36.76%) while reducing MDA accumulation by 48.27%. Cluster analysis indicated that JA concentrations between 0.1-1 μM were most effective in alleviating 1 mg/L Cr stress, while 0.5-1 μM JA optimally mitigated the effects under 10 mg/L Cr stress, and 0.5 μM JA was most beneficial under 30 mg/L Cr stress. JA was found to be essential for maintaining normal metabolic functions and alleviating Cr-induced damage in alfalfa. This study highlights the potential of jasmonic acid as an effective agent for mitigating chromium stress in alfalfa and advances the understanding of its role in plant stress physiology. These findings provide valuable insights for improving crop resilience in heavy metal-contaminated environments. Future research should focus on the broader application of JA in different plant species and under different environmental stress conditions.
重金属对土壤的污染日益严重，在中国内蒙古东部地区尤为如此，当地的铬铁矿开采因重金属残留导致了严重的环境问题。紫花苜蓿是该地区常见的饲料作物，被认为是一种有效的重金属污染修复作物。然而，仅靠紫花苜蓿在这种情况下的修复效果有限。茉莉酸（JA）作为一种主要的植物激素，在调节植物的各种生理过程中起着重要作用；因此，在本研究中，添加茉莉酸与紫花苜蓿协同修复铬污染。本研究的目的是探讨茉莉酸与紫花苜蓿联合修复铬污染的协同效应。具体而言，阐明了茉莉酸在不同铬浓度（1、10和30mg/L）下对紫花苜蓿生长和生理反应的调节作用。施用茉莉酸显著降低了紫花苜蓿幼苗中铬的积累，降低幅度为49.70%-87.37%，缓解效果取决于铬胁迫强度：在1mg/L铬浓度下，（0.1-1μM）茉莉酸分别降低了对根和茎的抑制作用74.86%和270.91%。在10mg/L铬浓度下，（0.5-1μM）茉莉酸抑制了氧化损伤，使HO和O水平分别降低了61.19%和14.91%。对于30mg/L铬浓度，0.5μM茉莉酸使总叶绿素合成最大化（增加2.03倍），根细胞活力提高（36.76%），同时使丙二醛积累降低48.27%。聚类分析表明，0.1-1μM的茉莉酸浓度在缓解1mg/L铬胁迫时最有效，而0.5-1μM的茉莉酸在缓解10mg/L铬胁迫时效果最佳，0.5μM的茉莉酸在30mg/L铬胁迫下最有益。研究发现茉莉酸对于维持紫花苜蓿的正常代谢功能和减轻铬诱导的损伤至关重要。本研究突出了茉莉酸作为缓解紫花苜蓿铬胁迫的有效试剂的潜力，并推进了对其在植物胁迫生理学中作用的理解。这些发现为提高重金属污染环境中作物的抗逆性提供了有价值的见解。未来的研究应侧重于茉莉酸在不同植物物种和不同环境胁迫条件下的更广泛应用。
