甜玉米维管束鞘细胞中外源小干扰RNA的检测及感染早期的RNA干扰动态

Detection of exogenous siRNA inside sweet corn bundle sheath cells and the RNAi dynamics in the early stage of infection.

作者信息

Balassa Kinga, Balassa György, Almási Asztéria, Visnovitz Tamás, Rudnóy Szabolcs

机构信息

Doctoral School of Biology, Institute of Biology, Eötvös Loránd University, Budapest, 1117 Hungary.

Department of Plant Physiology and Molecular Plant Biology, Faculty of Science, Eötvös Loránd University, Budapest, 1117 Hungary.

出版信息

Physiol Mol Biol Plants. 2024 Aug;30(8):1265-1276. doi: 10.1007/s12298-024-01500-2. Epub 2024 Aug 14.

DOI:10.1007/s12298-024-01500-2

PMID:39184556

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11341793/

Abstract

UNLABELLED

(MDMV) is one of the most serious viruses of sweet corn. Utilising the process of RNA interference, the exogenous introduction of small RNA molecules mimicking virus-derived small interfering RNA (siRNA) into the plant prior to infection triggers the antiviral RNA silencing effect, thereby promoting more effective antiviral protection. Hence, a treatment with MDMV-derived small RNA was applied to sweet corn plants one day before MDMV virus inoculation. ALEXA FLUOR®488 fluorophore-bound exogenous siRNA was successfully detected inside intact sweet corn cells using confocal fluorescence microscopy. Furthermore, it was demonstrated that the exogenous siRNA treatment led to a notable upregulation of the , , , , , , , , and genes within 24 h of the treatment. Overall, exogenous siRNA treatment resulted in better virus control of infected sweet corn plants, as indicated by the lower viral RNA and coat protein levels compared to the infected group without pre-treatment.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-024-01500-2.

摘要

未标记

玉米褪绿斑驳病毒（MDMV）是甜玉米最严重的病毒之一。利用RNA干扰过程，在感染前将模仿病毒衍生小干扰RNA（siRNA）的小RNA分子外源导入植物中，可触发抗病毒RNA沉默效应，从而促进更有效的抗病毒保护。因此，在接种MDMV病毒前一天，对甜玉米植株进行了MDMV衍生小RNA处理。使用共聚焦荧光显微镜在完整的甜玉米细胞内成功检测到了与ALEXA FLUOR®488荧光团结合的外源siRNA。此外，结果表明，外源siRNA处理在处理后24小时内导致了、、、、、、、和基因的显著上调。总体而言，与未进行预处理的感染组相比，外源siRNA处理使感染的甜玉米植株对病毒的控制效果更好，表现为病毒RNA和外壳蛋白水平较低。