Pinto Ana, da Silva Daniela Correia, Cardoso Ana, Fernandes Fátima, Soares Cristiano, Valentão Patrícia, Fidalgo Fernanda, Teixeira Jorge
GreenUPorto - Centro de Investigação em Produção Agroalimentar Sustentável - Inov4Agro & Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, Portugal.
REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
Physiol Mol Biol Plants. 2024 Aug;30(8):1353-1362. doi: 10.1007/s12298-024-01496-9. Epub 2024 Aug 2.
Progesterone (PROG) has been detected at various concentrations in the environment and has adverse effects on humans and wildlife. This work evaluated the impact of PROG in L. plants, its removal capacity, and how 2,4-epibrassinolide (24-EBL) affects this process. Three treatments were used: (1) control, (2) irrigation with 0.8 µM PROG, and (3) treatment with 0.8 µM PROG after a pre-treatment with a foliar application of 1 µM 2,4-EBL (PROG/24EBL). After 20 days of treatment, no PROG was detected in the nutrient solution or plant tissues, indicating that the PROG was removed and metabolized. Lipid peroxidation significantly decreased in response to PROG in shoots and roots, and this effect was even more significant for both organs of the PROG/24EBL plants. Additionally, both treatments in both organs showed a decrease in HO levels, and both steroid hormones increased the plants' antioxidant system at both the biochemical and gene expression levels. In conclusion, can swiftly remove PROG without affecting its growth, and the use of 24-EBL synergistically decreases oxidative damage by increasing the activity of the antioxidant system and enhancing plant PROG removal ability.
环境中已检测到不同浓度的孕酮（PROG），其对人类和野生动物具有不利影响。这项工作评估了PROG对番茄植株的影响、其去除能力，以及2,4-表油菜素内酯（24-EBL）如何影响这一过程。采用了三种处理方式：（1）对照，（2）用0.8 µM PROG灌溉，（3）在叶面喷施1 µM 2,4-EBL预处理后用0.8 µM PROG处理（PROG/24EBL）。处理20天后，在营养液或植物组织中未检测到PROG，表明PROG已被去除并代谢。PROG处理使地上部和根部的脂质过氧化显著降低，对于PROG/24EBL处理的植株，地上部和根部的这种影响更为显著。此外，两种处理的地上部和根部HO水平均降低，两种甾体激素在生化和基因表达水平上均增强了植物的抗氧化系统。总之，番茄能够迅速去除PROG而不影响其生长，24-EBL的使用通过增加抗氧化系统的活性和增强植物去除PROG的能力，协同降低了氧化损伤。
