Jafari Sorush, Saboori Masih, Ghasemi Sorayya
Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Neurosurgery, School of Medicine Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Mol Cell Med. 2024;13(2):160-170. doi: 10.22088/IJMCM.BUMS.13.2.160.
Glioblastoma multiforme (GBM) is an aggressive cancer with a poor prognosis. Inflammation and angiogenesis are important processes in GBM that are interrelated. In this study, bioinformatic investigations were performed to detect common and key genes in the inflammatory and angiogenesis pathways of GBM. Additionally, relevant long non-coding RNAs (lncRNAs) were recognized as important gene regulators. Consequently, real-time PCR and correlation analyses were used to investigate changes in gene and lncRNA expression levels and explain their relationship. RELA emerged as a common key gene in these biological processes. LINC01366 and LINC01433 were identified as putative RELA regulators in different metabolic pathways using computational assays. According to our findings, the expression levels of RELA, LINC01366 and LINC01433 were found to be significantly upregulated in GBM samples. Correlational studies revealed a significant positive relationship of gene expressions between LINC01366 and LINC01433, indicating that they may have a coordinated effect on GBM biology. Nevertheless, there was no significant correlation between these lncRNAs and RELA. The current study highlights the high expression of LINC01366 and LINC01433 in GBM and emphasizes the importance of studying lncRNAs as putative regulators in the pathophysiology of GBM. Further research is needed to clarify their specific functions, in particular the associated inflammatory and angiogenesis pathways.
多形性胶质母细胞瘤（GBM）是一种侵袭性癌症，预后较差。炎症和血管生成是GBM中相互关联的重要过程。在本研究中，进行了生物信息学研究，以检测GBM炎症和血管生成途径中的常见关键基因。此外，相关的长链非编码RNA（lncRNA）被认为是重要的基因调节因子。因此，采用实时PCR和相关性分析来研究基因和lncRNA表达水平的变化，并解释它们之间的关系。RELA成为这些生物学过程中的一个常见关键基因。使用计算分析方法，LINC01366和LINC01433被确定为不同代谢途径中假定的RELA调节因子。根据我们的研究结果，发现RELA、LINC01366和LINC01433的表达水平在GBM样本中显著上调。相关性研究揭示了LINC01366和LINC01433之间基因表达的显著正相关，表明它们可能对GBM生物学具有协同作用。然而，这些lncRNA与RELA之间没有显著相关性。当前的研究突出了LINC01366和LINC01433在GBM中的高表达，并强调了研究lncRNA作为GBM病理生理学中假定调节因子的重要性。需要进一步的研究来阐明它们的具体功能，特别是相关的炎症和血管生成途径。
