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纳米比亚多岩石山顶土壤放线菌的生化特性及其生物活性代谢产物对抗人类呼吸道病原体的拮抗作用分析。

Biochemical characterization of actinomycete from Namibia rocky crest mountainous soil and analyzing their bioactive metabolites for antagonistic effect against human respiratory pathogens.

机构信息

Department of Human, Biological and Translational Medical Sciences, University of Namibia, Private Bag 13301, Mandume Ndemufayo Avenue, Pionierspark, Windhoek, Namibia.

出版信息

Pan Afr Med J. 2024 May 14;48:12. doi: 10.11604/pamj.2024.48.12.33596. eCollection 2024.

DOI:10.11604/pamj.2024.48.12.33596
PMID:39184844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11343499/
Abstract

INTRODUCTION

the present study aimed at isolating and characterizing actinomycete from unexplored Windhoek rocky crest mountainous soil and extracting bioactive metabolites as possible therapeutics against common life-threatening Streptococcus pneumonia (S. pneumonia) and Stachybotrys chartarum (S. chartarum).

METHODS

chemotaxonomy and biochemical methods were used to identify the isolates. The solvent extraction method was used to extract bioactive compounds. Agar overlay and disc diffusion methods were used to determine the antimicrobial activity of isolates and extracted bioactive metabolites against S. pneumonia and S. chartarum. The antioxidant activity of the extracted bioactive metabolites was determined using 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method with ascorbic acid as a positive control. Comparison between groups was done using a Two-way ANOVA, followed by Bonferroni post-test.

RESULTS

three distinct isolates from 3 soil samples were identified on starch casein agar and distinguished using biochemical tests. All three isolates showed strong inhibitory activity against S. pneumonia with average growth inhibition zones between 18.0±1.00 and 27±0.00 mm p< 0.005. All isolates showed potent inhibitory activity against S. chartarum with the average inhibition zones ranging between 42.0±1.00 and 48±0.00 mm, p< 0.005. The chloroform extracts showed potent DPPH activity of up to 73± 1.41%.

CONCLUSION

growth conditions and extraction solvents can influence the antimicrobial and antioxidant properties of bioactive metabolites.

摘要

简介

本研究旨在从未开发的温得和克多岩石山顶土壤中分离和鉴定放线菌，并提取生物活性代谢物，作为治疗常见危及生命的肺炎链球菌（S. pneumonia）和构巢曲霉（S. chartarum）的潜在疗法。

方法

采用化学分类学和生化方法对分离物进行鉴定。采用溶剂萃取法提取生物活性化合物。琼脂覆盖法和圆盘扩散法测定分离物和提取的生物活性代谢物对 S. pneumonia 和 S. chartarum 的抗菌活性。采用 2.2-二苯基-1-苦基肼（DPPH）自由基清除法测定提取的生物活性代谢物的抗氧化活性，以抗坏血酸为阳性对照。采用双因素方差分析，然后进行 Bonferroni 事后检验比较组间差异。

结果

从 3 个土壤样本中分离出 3 个不同的分离株，在淀粉酪蛋白琼脂上进行鉴定，并通过生化试验进行区分。所有 3 个分离株对 S. pneumonia 的生长抑制活性均较强，平均抑菌环直径为 18.0±1.00 至 27±0.00mm，p<0.005。所有分离株对 S. chartarum 的抑制活性均较强，平均抑菌环直径为 42.0±1.00 至 48±0.00mm，p<0.005。氯仿提取物表现出高达 73±1.41%的 DPPH 活性。

结论

生长条件和提取溶剂会影响生物活性代谢物的抗菌和抗氧化特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/7756184d096c/PAMJ-48-12-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/9470c65e9986/PAMJ-48-12-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/ea47e854789a/PAMJ-48-12-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/9182986cfc5c/PAMJ-48-12-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/d560cdeff9cf/PAMJ-48-12-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/2f2900d678cd/PAMJ-48-12-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/7756184d096c/PAMJ-48-12-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/9470c65e9986/PAMJ-48-12-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/ea47e854789a/PAMJ-48-12-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/9182986cfc5c/PAMJ-48-12-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/d560cdeff9cf/PAMJ-48-12-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/2f2900d678cd/PAMJ-48-12-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e85/11343499/7756184d096c/PAMJ-48-12-g006.jpg

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