Shoraba Mostafa, Shoulah Salma A, Arnaout Faysal, Selim Abdelfattah
Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine Benha University, Toukh 13736, Egypt.
Vet Med Int. 2024 Aug 16;2024:6550276. doi: 10.1155/2024/6550276. eCollection 2024.
Surra caused by () is widely distributed and has significant impact on equine sector and international trades. However, there are no available data about the genetic characterization of this parasite in horses in Egypt. So, the goal of this study was to study the molecular characterization of in horses and determine the changes in hematological parameters and oxidative stress associated with infection. A total of 12 horses were examined using PCR targeting RoTat 1.2 VSG gene, and we evaluated the changes in hematological and oxidative stress between infected and healthy animals. The results revealed a notable reduction in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) levels in the infected horses, as compared to the control healthy group. In contrast, the infected group showed a substantial increase in mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), and mean corpuscular volume (MCV). In addition, the infected group exhibited monocytopenia, eosinopenia, and notable lymphocytopenia. Regarding oxidative stress profile, the infected horses showed decreased levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and antioxidant capacity (TAC) compared to the control group. Moreover, the PCR assay targeting RoTat 1.2 VSG gene revealed positive specific band (205 bp) in all examined samples for and the phylogenetic analysis demonstrated that strain of this study is closely related to isolate in horses from India (MT501210) while showed difference from sequences of other species. The results emphasize the changes in blood composition and the body's response to oxidative stress caused by infection in horses.
由（）引起的苏拉病分布广泛，对马产业和国际贸易有重大影响。然而，在埃及的马匹中，尚无关于这种寄生虫基因特征的可用数据。因此，本研究的目的是研究马匹中（寄生虫名称未完整给出）的分子特征，并确定与（寄生虫感染）相关的血液学参数变化和氧化应激。总共对12匹马进行了针对RoTat 1.2 VSG基因的PCR检测，并评估了感染动物与健康动物之间血液学和氧化应激的变化。结果显示，与健康对照组相比，感染马匹的红细胞（RBC）、血细胞比容（HCT）和血红蛋白（Hb）水平显著降低。相反，感染组的平均红细胞血红蛋白浓度（MCHC）、平均红细胞血红蛋白（MCH）和平均红细胞体积（MCV）大幅增加。此外，感染组出现单核细胞减少、嗜酸性粒细胞减少和明显的淋巴细胞减少。关于氧化应激情况，与对照组相比，感染马匹的谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗氧化能力（TAC）水平降低。此外，针对RoTat 1.2 VSG基因的PCR检测在所有检测样本中均显示出针对（寄生虫名称未完整给出）的阳性特异性条带（205 bp），系统发育分析表明，本研究中的菌株与来自印度的马匹中的（寄生虫名称未完整给出）分离株密切相关，同时与其他物种的序列存在差异。结果强调了马匹感染（寄生虫名称未完整给出）后血液成分的变化以及机体对氧化应激的反应。
