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2
Modulating Substrate Specificity of sp. Histamine Dehydrogenase through Protein Engineering for Food Quality Applications.通过蛋白质工程调节 sp. 组氨酸脱氢酶的底物特异性，以应用于食品质量。
Molecules. 2023 Apr 26;28(9):3748. doi: 10.3390/molecules28093748.
3
Validation Study of BioSystems® Y15 Histamine Dehydrogenase Kit for the Detection of Histamine in Fish and Fishery Products: AOAC Performance Tested MethodSM 072001.BioSystems® Y15 组胺脱氢酶试剂盒用于检测鱼类和渔业产品中组胺的验证研究：AOAC 性能测试方法 SM 072001。
J AOAC Int. 2021 Jun 12;104(3):693-711. doi: 10.1093/jaoacint/qsaa139.
4
Validation of the R-Biopharm AG RIDASCREEN® Histamine (Enzymatic) Kit.
J AOAC Int. 2019 Sep 1;102(5):1472-1491. doi: 10.1093/jaoac/102.5.1472.
5
The biogenic amines putrescine and cadaverine show in vitro cytotoxicity at concentrations that can be found in foods.腐胺和尸胺在体外具有细胞毒性，其浓度在食物中可以检测到。
Sci Rep. 2019 Jan 15;9(1):120. doi: 10.1038/s41598-018-36239-w.
6
Biogenic Amine Production by Lactic Acid Bacteria: A Review.乳酸菌产生生物胺的研究综述
Foods. 2019 Jan 7;8(1):17. doi: 10.3390/foods8010017.
7
Recent trends in the determination of biogenic amines in fermented beverages - A review.发酵饮料中生物胺测定的最新趋势——综述
Anal Chim Acta. 2016 Oct 5;939:10-25. doi: 10.1016/j.aca.2016.07.045. Epub 2016 Aug 3.
8
Construction of a bifunctional enzyme fusion for the combined determination of biogenic amines in foods.用于食品中生物胺联合测定的双功能酶融合体的构建。
J Agric Food Chem. 2013 Sep 25;61(38):9118-24. doi: 10.1021/jf403044m. Epub 2013 Sep 13.
9
Characterization of amine oxidases from Arthrobacter aurescens and application for determination of biogenic amines.从节杆菌属中分离的胺氧化酶的特性及其在生物胺测定中的应用。
World J Microbiol Biotechnol. 2013 Apr;29(4):673-82. doi: 10.1007/s11274-012-1223-y. Epub 2012 Dec 7.
10
Enzyme sensor array for the determination of biogenic amines in food samples.用于测定食品样品中生物胺的酶传感器阵列。
Anal Bioanal Chem. 2002 Jan;372(2):276-83. doi: 10.1007/s00216-001-1130-9. Epub 2001 Dec 15.

基于胺氧化酶-过氧化物酶偶联反应，以抗坏血酸作为羟基清除剂对生物源单胺进行可视化检测。

Visual detection of biogenic monoamines based on amine oxidase-peroxidase coupling reaction using ascorbic acid as HO scavenger.

作者信息

Kim Young-Chang, Ha Jun-Mo, Lee Jaeick, Kim Young-Wan

机构信息

Department of Food and Biotechnology, Korea University, Sejong, 30019 Republic of Korea.

出版信息

Food Sci Biotechnol. 2024 Mar 12;33(12):2755-2760. doi: 10.1007/s10068-023-01511-x. eCollection 2024 Sep.

DOI:10.1007/s10068-023-01511-x
PMID:39184985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11339208/
Abstract

This study represents a visual detection for total biogenic monoamines with naked eye as a simple and rapid semi-quantitative method for biogenic amine monitoring. The equivalent reaction of HO with ascorbic acid resulted in color development by an amine oxidase-peroxidase coupling reaction in the samples containing the biogenic monoamines higher than the subjected ascorbic acid by 10 μM. Upon employing the commercial doenjang extracts as a model food, an additional heating step was requested, and the expected ranges for the biogenic monoamines from 360 to 480 μM covered the real contents of the samples (360.2-407.3 μM). Therefore, this visual detection method makes it possible to decide with naked eye whether the sample contains the biogenic monoamines higher than the ascorbic acid supplemented as much as a control level on manufacturing sites without instrumental analysis.

摘要

本研究提出了一种用于检测总生物源单胺的肉眼视觉检测方法，作为一种简单快速的生物胺监测半定量方法。在含有比受试抗坏血酸高10μM生物源单胺的样品中，HO与抗坏血酸的等效反应通过胺氧化酶-过氧化物酶偶联反应导致显色。以市售豆酱提取物作为模型食品时，需要额外的加热步骤，生物源单胺的预期范围为360至480μM，涵盖了样品的实际含量（360.2-407.3μM）。因此，这种视觉检测方法使得无需仪器分析，仅凭肉眼就能判断样品中生物源单胺的含量是否高于在生产现场作为对照水平添加的抗坏血酸。