基于胺氧化酶-过氧化物酶偶联反应，以抗坏血酸作为羟基清除剂对生物源单胺进行可视化检测。
Visual detection of biogenic monoamines based on amine oxidase-peroxidase coupling reaction using ascorbic acid as HO scavenger.
作者信息
Kim Young-Chang, Ha Jun-Mo, Lee Jaeick, Kim Young-Wan
机构信息
Department of Food and Biotechnology, Korea University, Sejong, 30019 Republic of Korea.
出版信息
Food Sci Biotechnol. 2024 Mar 12;33(12):2755-2760. doi: 10.1007/s10068-023-01511-x. eCollection 2024 Sep.
This study represents a visual detection for total biogenic monoamines with naked eye as a simple and rapid semi-quantitative method for biogenic amine monitoring. The equivalent reaction of HO with ascorbic acid resulted in color development by an amine oxidase-peroxidase coupling reaction in the samples containing the biogenic monoamines higher than the subjected ascorbic acid by 10 μM. Upon employing the commercial doenjang extracts as a model food, an additional heating step was requested, and the expected ranges for the biogenic monoamines from 360 to 480 μM covered the real contents of the samples (360.2-407.3 μM). Therefore, this visual detection method makes it possible to decide with naked eye whether the sample contains the biogenic monoamines higher than the ascorbic acid supplemented as much as a control level on manufacturing sites without instrumental analysis.
本研究提出了一种用于检测总生物源单胺的肉眼视觉检测方法，作为一种简单快速的生物胺监测半定量方法。在含有比受试抗坏血酸高10μM生物源单胺的样品中，HO与抗坏血酸的等效反应通过胺氧化酶-过氧化物酶偶联反应导致显色。以市售豆酱提取物作为模型食品时，需要额外的加热步骤，生物源单胺的预期范围为360至480μM，涵盖了样品的实际含量（360.2-407.3μM）。因此，这种视觉检测方法使得无需仪器分析，仅凭肉眼就能判断样品中生物源单胺的含量是否高于在生产现场作为对照水平添加的抗坏血酸。
Zotero 插件