Dairy Research Institute, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.
Sci Rep. 2019 Jan 15;9(1):120. doi: 10.1038/s41598-018-36239-w.
Putrescine and cadaverine are among the most common biogenic amines (BA) in foods, but it is advisable that their accumulation be avoided. Present knowledge about their toxicity is, however, limited; further research is needed if qualitative and quantitative risk assessments for foods are to be conducted. The present work describes a real-time analysis of the cytotoxicity of putrescine and cadaverine on intestinal cell cultures. Both BA were cytotoxic at concentrations found in BA-rich foods, although the cytotoxicity threshold for cadaverine was twice that of putrescine. Their mode of cytotoxic action was similar, with both BA causing cell necrosis; they did not induce apoptosis. The present results may help in establishing legal limits for both putrescine and cadaverine in food.
腐胺和尸胺是食品中最常见的生物胺（BA）之一，但最好避免它们的积累。然而，目前对它们毒性的了解是有限的；如果要对食品进行定性和定量风险评估，需要进一步研究。本工作描述了腐胺和尸胺对肠细胞培养物细胞毒性的实时分析。在 BA 丰富的食品中发现的浓度下，两种 BA 均具有细胞毒性，尽管尸胺的细胞毒性阈值是腐胺的两倍。它们的细胞毒性作用模式相似，两种 BA 都导致细胞坏死；它们不会诱导细胞凋亡。本研究结果可能有助于确定食品中腐胺和尸胺的法定限量。
