Park Jin-Wook, Lee Gayeong, Shin Jae-Wook, Yun Choong-In, Kim Young-Jun
Department of Food Science and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
Korea Advanced Food Research Institute, Uiwang, 16001 Republic of Korea.
Food Sci Biotechnol. 2024 Mar 18;33(12):2747-2754. doi: 10.1007/s10068-024-01544-w. eCollection 2024 Sep.
This study reports the improvement and validation of a colorimetric method to quantify polysorbates (20, 60, 65, and 80) in food by measuring absorbance at 620 nm using ultraviolet-visible spectrophotometry. The method was validated for linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, accuracy, and measurement uncertainty. The coefficient of determination was linear ( ≥ 0.9991) over the measured concentration range of 50-1000 mg/L. The LOD and LOQ were 2.3-4.9 and 7.0-15.0 mg/kg, respectively. Intra-day and inter-day accuracy and precision were 91.9-104.1% and 0.1-1.1% RSD, and 91.6-103.8% and 0.4-5.0% RSD, respectively. The result of inter-laboratory recovery was 90.9-99.8% and the measurement uncertainty was < 16% with the compliance of the CODEX recommendation. Sauce, bread, whipped cream, rice cake, ice cream, and various other polysorbate-labeled food products ( = 229, detection range; N.D.-16,442.3 mg/kg) distributed in Korea were analyzed to confirm the applicability of the analytical method.
The online version contains supplementary material available at 10.1007/s10068-024-01544-w.
本研究报告了一种比色法的改进与验证，该方法通过紫外可见分光光度法测量620nm处的吸光度来定量食品中的聚山梨酯（20、60、65和80）。该方法在线性、检测限（LOD）、定量限（LOQ）、精密度、准确度和测量不确定度方面进行了验证。在50 - 1000mg/L的测量浓度范围内，测定系数呈线性（≥0.9991）。LOD和LOQ分别为2.3 - 4.9mg/kg和7.0 - 15.0mg/kg。日内和日间准确度和精密度分别为91.9 - 104.1%和0.1 - 1.1%RSD，以及91.6 - 103.8%和0.4 - 5.0%RSD。实验室间回收率结果为90.9 - 99.8%，测量不确定度<16%，符合食品法典委员会的建议。对在韩国销售的酱汁、面包、鲜奶油、米糕、冰淇淋和其他各种标注了聚山梨酯的食品（n = 229，检测范围；未检出 - 16,442.3mg/kg）进行了分析，以确认该分析方法的适用性。
在线版本包含可在10.1007/s10068 - 024 - 01544 - w获取的补充材料。
