Lim SuJi, Yun Choong-In, Song Dahyun, Kang Seok-Seong, Kim Young-Jun
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Seoul, Korea.
Food Sci Biotechnol. 2022 May 27;31(9):1145-1155. doi: 10.1007/s10068-022-01099-8. eCollection 2022 Aug.
Oat contains a variety of phenolic compounds, including avenanthramides, which are found only in oats. This study was conducted to establish the quantitative analysis of seven phenolic compounds in oat powders and sprouted oat powders and validate an efficient high-performance liquid chromatography (HPLC) method. All calibration curves represented good linearity ( ≥ 0.9997) in the concentration range (0.5-50 mg/kg) with LOD and LOQ of 0.01-0.21 and 0.02-0.64 mg/kg, respectively. Intra-day accuracy (%) and precision (%RSD) were 90.7-103.8% and 1.5-4.9%, respectively. Inter-day accuracy (%) and precision (%RSD) were 90.4-107.9% and 2.2-4.8%, respectively. Moreover, relative expanded measurement uncertainty results complied with CODEX guideline. In addition, selected oat and sprouted oat powder extracts showed anti-microbial activities against O157:H7. These results demonstrated that this HPLC method is suitable for the qualification and quantification of seven phenolic compounds in oat and sprouted oat.
The online version contains supplementary material available at 10.1007/s10068-022-01099-8.
燕麦含有多种酚类化合物，包括仅存在于燕麦中的燕麦酰胺。本研究旨在建立燕麦粉和发芽燕麦粉中七种酚类化合物的定量分析方法，并验证一种高效液相色谱（HPLC）方法的有效性。所有校准曲线在浓度范围（0.5 - 50 mg/kg）内均呈现良好的线性关系（≥0.9997），检测限（LOD）和定量限（LOQ）分别为0.01 - 0.21和0.02 - 0.64 mg/kg。日内准确度（%）和精密度（%RSD）分别为90.7 - 103.8%和1.5 - 4.9%。日间准确度（%）和精密度（%RSD）分别为90.4 - 107.9%和2.2 - 4.8%。此外，相对扩展测量不确定度结果符合食品法典委员会指南。此外，所选燕麦和发芽燕麦粉提取物对O157:H7显示出抗菌活性。这些结果表明，该HPLC方法适用于燕麦和发芽燕麦中七种酚类化合物的定性和定量分析。
在线版本包含可在10.1007/s10068 - 022 - 01099 - 8获取的补充材料。
