Fernandes Ana Catarina da Silva Teixeira, da Silva Daniela Maria Carneiro, Queiroga Felisbina Luísa, Silvestre-Ferreira Ana C
Department of Clinical Biology, School of Life and Environmental Sciences, University of Trás-os-Montes and Alto Douro, Quinta de Prados, Vila Real, Portugal.
Laboratory of Animal Health and Food Safety (Segalab), Argivai, Póvoa de Varzim, Portugal.
Vet World. 2024 Jul;17(7):1591-1595. doi: 10.14202/vetworld.2024.1591-1595. Epub 2024 Jul 24.
Anemia, a clinical condition characterized by reduced erythrocytes, is often observed in cats. Regeneration indicates that the bone marrow can respond appropriately to anemia. The absolute reticulocyte count is the reference for differentiating regenerative and non-regenerative anemia, while red blood cell (RBC) indices and morphology provide supplementary information. This study aimed to identify anemia types and establish the most reliable RBC indices and morphology methods in agreement with the reference method.
One hundred forty-five cases of cat anemia were prospectively classified using two methods: RBC indices and RBC morphology, and subsequently compared with the absolute reticulocyte count.
Based on RBC indices assessment, 27 cases (19%) exhibited regenerative anemia. Based on RBC morphology, 29 (20%) cases were identified as having regenerative anemia. Using the reticulocyte absolute count as a reference method, 34 (23.4%) cases of regenerative anemia were identified. The findings indicated that RBC indices and RBC morphology did not align in evaluating medullary regeneration and that there is a good degree of agreement between RBC morphology assessment and the reticulocyte absolute count in identifying regenerative anemias.
Blood smear analysis of RBC morphology was more dependable for classifying regenerative anemia than RBC indices. Further studies should be conducted with a larger number of animals and that allow the identification of the cause of anemia and the monitoring of the animal.
贫血是一种以红细胞减少为特征的临床病症，在猫中较为常见。再生表明骨髓能够对贫血做出适当反应。绝对网织红细胞计数是区分再生性贫血和非再生性贫血的参考指标，而红细胞（RBC）指数和形态学提供补充信息。本研究旨在确定贫血类型，并建立与参考方法一致的最可靠的红细胞指数和形态学方法。
前瞻性地采用两种方法对145例猫贫血病例进行分类：红细胞指数和红细胞形态学，随后与绝对网织红细胞计数进行比较。
基于红细胞指数评估，27例（19%）表现为再生性贫血。基于红细胞形态学，29例（20%）被确定为再生性贫血。以网织红细胞绝对计数作为参考方法，确定了34例（23.4%）再生性贫血。结果表明，红细胞指数和红细胞形态学在评估骨髓再生方面不一致，且在识别再生性贫血方面，红细胞形态学评估与网织红细胞绝对计数之间具有较好的一致性。
红细胞形态学的血涂片分析在分类再生性贫血方面比红细胞指数更可靠。应使用更多动物进行进一步研究，以确定贫血原因并监测动物情况。
