Geriatrics Department, Huzhou Third Municipal Hospital, the Affiliated Hospital of Huzhou University, Zhejiang province, Huzhou 313000, China.
Department of Psychiatry, Huzhou Third Municipal Hospital, the Affiliated Hospital of Huzhou University, Zhejiang province, Huzhou 313000, China.
Biotechniques. 2024;76(7):333-341. doi: 10.1080/07366205.2024.2348347. Epub 2024 Jun 5.
MicroRNA (miRNA) has garnered considerable attention due to its diagnostic capabilities, such as in hypoxic cognitive impairment and cancers. However, the existing miRNA detection methods are commonly criticized for the drawbacks of low sensitivity and false-positive detection derived from interfering molecules. Here, we provide a novel, sensitive and portable method for miRNA detection by combining target identification based cyclization of padlocks, immobilized primer-based signal amplification and a personal glucose meter. The proposed method exhibits several advantages, including precise identification of specific sites, exceptional sensitivity and instrument-free feature. These attributes hold great promise for the diagnosis and clinical investigation of various diseases, such as cancer and hypoxic cognitive impairment, enabling a deeper understanding of their pathological and physiological aspects.
微 RNA（miRNA）因其在缺氧性认知障碍和癌症等方面的诊断能力而备受关注。然而，现有的 miRNA 检测方法通常因其灵敏度低和由干扰分子引起的假阳性检测而受到批评。在这里，我们提供了一种新颖、敏感和便携的 miRNA 检测方法，该方法结合了基于发夹环化的靶标识别、基于固定引物的信号放大和个人血糖仪。所提出的方法具有几个优点，包括对特定部位的精确识别、出色的灵敏度和无仪器的特点。这些特性为癌症和缺氧性认知障碍等各种疾病的诊断和临床研究提供了巨大的潜力，使人们能够更深入地了解它们的病理和生理方面。
