Docker Andrew, Min Tay Hui
Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK.
Chemistry. 2024 Dec 5;30(68):e202402844. doi: 10.1002/chem.202402844. Epub 2024 Oct 16.
Determining ion-pair affinities in heteroditopic receptor systems presents a persistent and significant challenge. The plethora of technical and experimental problems implicated in measuring ion-pair affinities have encouraged the use of several expedient experimental practices as a means of characterising ion-pair recognition behaviour. Exploiting a model heteroditopic receptor system, we interrogate the reliability of these methods and demonstrate that these commonly used techniques can be highly questionable and without extreme care can lead to incorrect conclusions.
在异双位点受体系统中测定离子对亲和力是一项长期存在且极具挑战性的任务。测量离子对亲和力涉及大量技术和实验问题，这促使人们采用多种便捷的实验方法来表征离子对识别行为。利用一个模型异双位点受体系统，我们探究了这些方法的可靠性，并证明这些常用技术可能存在很大问题，若不格外谨慎，可能会得出错误结论。
