Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Proc Natl Acad Sci U S A. 2024 Sep 3;121(36):e2405410121. doi: 10.1073/pnas.2405410121. Epub 2024 Aug 26.
The gut microbiome plays an important role in honeybee hormonal regulation and growth, but the underlying mechanisms are poorly understood. Here, we showed that the depletion of gut bacteria resulted in reduced expression of insulin-like peptide gene () in the head, accompanied by metabolic syndromes resembling those of Type 1 diabetes in humans: hyperglycemia, impaired lipid storage, and decreased metabolism. These symptoms were alleviated by gut bacterial inoculation. Gut metabolite profiling revealed that succinate, produced by Firm-5, played deterministic roles in activating gene expression and in regulating metabolism in honeybees. Notably, we demonstrated that succinate modulates host gene expression through stimulating gut gluconeogenesis, a mechanism resembling that of humans. This study presents evidence for the role of gut metabolite in modulating host metabolism and contributes to the understanding of the interactions between gut microbiome and bee hosts.
肠道微生物群在蜜蜂的激素调节和生长中起着重要作用，但相关的潜在机制仍不清楚。在这里，我们发现肠道细菌的耗竭导致头部胰岛素样肽基因 ()的表达减少，同时出现类似于人类 1 型糖尿病的代谢综合征：高血糖、脂质储存受损和代谢降低。这些症状可以通过肠道细菌接种得到缓解。肠道代谢产物分析表明，Firm-5 产生的琥珀酸在激活蜜蜂基因表达和调节代谢方面起着决定性作用。值得注意的是，我们证明琥珀酸通过刺激肠道糖异生来调节宿主基因表达，这一机制类似于人类。本研究为肠道代谢物在调节宿主代谢中的作用提供了证据，并有助于理解肠道微生物群与蜜蜂宿主之间的相互作用。
