一种用于准确、灵敏检测 miRNA-21 的三重信号放大策略。
A Triple Signal Amplification Strategy for Accurate and Ultrasensitive miRNA-21 Detection.
机构信息
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
出版信息
Anal Chem. 2024 Sep 10;96(36):14464-14470. doi: 10.1021/acs.analchem.4c02355. Epub 2024 Aug 26.
A triple signal amplification strategy was integrated with a built-in double electrode and external energy storage device to fabricate a novel self-powered biosensor for ultrasensitive detection of miRNA-21. Specifically, DNA tetrahedra and haripin2-glucose oxidase are modified on the surface of the biocathode and bioanode by catalytic hairpin assembly (CHA) to achieve dual signal amplification. Moreover, triple signal amplification is realized by including an external capacitor. Consequently, the as-constructed self-powered biosensor demonstrates a low detection limit of 0.06 fM toward the miRNA-21 assay within the range of 0.1 fM to 10 pM. This study presents a practical and sensitive approach to timely cancer detection.
采用内置双电极和外部储能装置集成三重信号放大策略，构建了一种新型自供电生物传感器，用于超灵敏检测 miRNA-21。具体来说，通过催化发夹组装（CHA）将 DNA 四面体和 haripin2-葡萄糖氧化酶修饰在生物阴极和生物阳极的表面，实现双重信号放大。此外，通过包含外部电容器实现三重信号放大。因此，所构建的自供电生物传感器在 0.1 fM 至 10 pM 的范围内对 miRNA-21 检测表现出 0.06 fM 的低检测限。本研究为癌症的及时检测提供了一种实用且灵敏的方法。
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