Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, China.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
Anal Chem. 2024 Sep 10;96(36):14508-14515. doi: 10.1021/acs.analchem.4c02751. Epub 2024 Aug 23.
An ultrasensitive self-powered biosensor is constructed for miRNA-21 detection based on Au nanoparticles @ Pd nanorings (Au NPs@Pd NRs) and catalytic hairpin assembly (CHA). The Au NPs@Pd NRs possess excellent electrical conductivity to improve the electron transfer rate and show good elimination of byproduct HO to assist glucose oxidase (GOD) to catalyze glucose; CHA is used as an amplification strategy to effectively enhance the sensitivity of the biosensor. To further amplify the output signal, a capacitor is integrated into the self-powered biosensor. With multiple signal amplification strategies, the self-powered biosensor possesses a linear range of 0.1-10 fM and a lower limit of detection (LOD) of 0.032 fM (/ = 3). In addition, the as-prepared self-powered biosensor displays potential applicability in the assay toward miRNA-21 in human serum samples.
构建了一种基于金纳米粒子@钯纳米环（Au NPs@Pd NRs）和催化发夹组装（CHA）的超灵敏自供电生物传感器，用于 miRNA-21 的检测。Au NPs@Pd NRs 具有优异的导电性，可提高电子转移速率，并显示出良好的消除副产物 HO 的能力，以协助葡萄糖氧化酶（GOD）催化葡萄糖；CHA 被用作放大策略，可有效提高生物传感器的灵敏度。为了进一步放大输出信号，将电容器集成到自供电生物传感器中。通过多种信号放大策略，自供电生物传感器具有 0.1-10 fM 的线性范围和 0.032 fM 的检测限（LOD）（/ = 3）。此外，所制备的自供电生物传感器在人血清样本中对 miRNA-21 的测定中表现出潜在的适用性。
