Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Horticulture, Faculty of Agriculture, Kasetsart University, Chatuchak, Bangkok10900, Thailand.
J Microbiol Biotechnol. 2024 Sep 28;34(9):1857-1866. doi: 10.4014/jmb.2401.01034. Epub 2024 Jul 19.
Endophytic fungi have been shown to synthesize bioactive secondary metabolites, some of which promote plant growth through various mechanisms. In our previous study, endophytic fungi were isolated from mango trees ( L.). The present study examined fifty endophytic fungal isolates for mineral solubilization activity, ammonia production, and siderophore production. It was shown that these isolates could produce phytohormones indole-3-acetic acid and gibberellic acid, as well as inhibit plant pathogens, specifically and . The results showed that all the isolated fungal endophytes exhibited various activities. Based on the findings, two fungal endophytes- CY.OS 13 and CY.OS 144-were selected for dual inoculation in chili plants under pot-scale conditions to investigate their potential to improve growth-related traits such as seed germination, shoot and root length, biomass, and chlorophyll content. Seed treated with CY.OS 13 and/or CY.OS 144 showed a significant ( < 0.05) increase in seed germination and growth parameters of chili plants grown under pot-scale conditions. Particularly, chili plants whose seeds were injected with a combination of the two selected endophytic fungi showed the highest plant development traits. Therefore, the selected endophytic fungi have the potential to be used as biofertilizers, especially when combined. They could eventually replace chemical fertilizers because they are environmentally friendly, beneficial to humans, and can even promote sustainable agriculture.
内生真菌已被证明能合成生物活性的次生代谢物，其中一些通过各种机制促进植物生长。在我们之前的研究中，从芒果树（L.）中分离出内生真菌。本研究检测了 50 株内生真菌分离物的溶磷活性、氨产生和铁载体产生。结果表明，这些分离物可以产生植物激素吲哚-3-乙酸和赤霉素，以及抑制植物病原菌，特别是 和 。结果表明，所有分离的内生真菌都表现出各种活性。基于这些发现，选择了两种真菌内生菌- CY.OS 13 和 CY.OS 144-在盆栽条件下对辣椒植物进行双重接种，以研究它们在改善种子发芽、茎和根长、生物量和叶绿素含量等生长相关特性方面的潜力。用 CY.OS 13 和/或 CY.OS 144 处理的种子在盆栽条件下显著（<0.05）增加了辣椒种子的发芽和生长参数。特别是，用两种选定的内生真菌组合注射种子的辣椒植物表现出最高的植物发育特性。因此，所选的内生真菌具有作为生物肥料的潜力，特别是当它们结合使用时。它们最终可以取代化肥，因为它们对环境友好，对人类有益，甚至可以促进可持续农业。
