Jiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, 332005, Jiangxi, China.
Jiujiang Key Laboratory of Rare Disease ResearchSchool of Basic Medical Sciences, Jiujiang University, Jiujiang, 332005, China.
Sci Rep. 2024 Aug 26;14(1):19787. doi: 10.1038/s41598-024-70867-9.
Circular RNAs (circRNAs) in controlling gene expression have been highlighted by increasing evidence, and their dysregulation has been linked to various diseases. However, the limited role of circRNAs in the adipogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) has been explored. High-throughput sequencing of circRNA was carried out on BMSCs and AD induction 7d BMSCs. Then a substantial upregulation of circNDUFA13 was detected among circRNAs in AD induction 7d BMSCs. We found that the adipogenic differentiation of BMSCs was positively linked with circNDUFA13 expression levels. Adipogenesis in BMSCs was effectively inhibited by circNDUFA13 knockdown, whereas overexpression of circNDUFA13 promoted adipogenesis. It was noted that circNDUFA13 regulated the adipogenic differentiation of BMSCs by directly interacting with the signal transducer and activator of transcription 3 (STAT3), which activates CEBPβ transcription. The in vitro model also validated the in vivo findings. our results suggest that circNDUFA13 controlled the adipogenic differentiation of BMSCs by targeting STAT3 and CEBPβ activation.
环状 RNA（circRNAs）在基因表达调控中的作用已被大量证据所强调，其失调与多种疾病有关。然而，circRNAs 在骨髓间充质干细胞（BMSCs）成脂分化中的作用有限。对 BMSCs 和 AD 诱导 7d BMSCs 进行了 circRNA 的高通量测序。然后在 AD 诱导 7d BMSCs 中的 circRNAs 中检测到 circNDUFA13 的大量上调。我们发现 BMSCs 的成脂分化与 circNDUFA13 表达水平呈正相关。circNDUFA13 的敲低有效抑制了 BMSCs 的成脂分化，而 circNDUFA13 的过表达则促进了成脂分化。值得注意的是，circNDUFA13 通过直接与信号转导和转录激活因子 3（STAT3）相互作用来调节 BMSCs 的成脂分化，从而激活 CEBPβ 转录。体外模型也验证了体内研究结果。我们的结果表明，circNDUFA13 通过靶向 STAT3 和 CEBPβ 的激活来控制 BMSCs 的成脂分化。
