Wang Jinglin, Qin Xueqian, Kong Bin, Ren Haozhen
Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine School of Pharmacy Nanjing University of Chinese Medicine Nanjing Jiangsu China.
State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing China.
Smart Med. 2022 Dec 16;1(1):e20220002. doi: 10.1002/SMMD.20220002. eCollection 2022 Dec.
Decellularized scaffolds have a demonstrated value in liver tissue engineering. Challenges in this area are focused on effectively eliminating the biological rejection of scaffolds and finding a suitable liver cell source. Here, inspired by the natural microstructure of hepatic lobules, we present a novel decellularized celery-derived scaffold cultured with human-induced pluripotent stem cell-derived hepatocytes (hiPSC-Heps) bioengineering liver tissue construction. Because of the natural hollow channels, interconnected porous structures, and excellent physicochemical characterization of the decellularized celery-derived scaffold, the resultant bioengineering liver tissue can maintain the hiPSC-Heps viability and the hepatic functions in the in vitro cultures. Based on this bioengineering liver tissue, we have demonstrated its good biocompatibility and the significantly higher expressions of albumin (ALB) and periodic acid-schiff stain (PAS) when it was implanted in nude mice. These remarkable properties endow the hiPSC-Heps integrated decellularized celery scaffolds system with promising prospects in the field of liver transplantation and other regeneration medicine.
去细胞支架在肝组织工程中已显示出价值。该领域的挑战集中在有效消除支架的生物排斥反应以及寻找合适的肝细胞来源。在此，受肝小叶自然微观结构的启发，我们提出了一种新型的去细胞芹菜衍生支架，其与人诱导多能干细胞衍生的肝细胞（hiPSC-Heps）一起培养用于生物工程肝组织构建。由于去细胞芹菜衍生支架具有天然的中空通道、相互连接的多孔结构以及出色的物理化学特性，由此产生的生物工程肝组织在体外培养中能够维持hiPSC-Heps的活力和肝功能。基于这种生物工程肝组织，我们已经证明其具有良好的生物相容性，并且在植入裸鼠体内时白蛋白（ALB）和过碘酸希夫染色（PAS）的表达显著更高。这些显著特性使hiPSC-Heps整合去细胞芹菜支架系统在肝移植和其他再生医学领域具有广阔前景。
