Department of Surgery, Keio University School of Medicine, Shinanomachi 35, Shinjuku 162-8666, Japan.
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita 565-0871, Japan.
Cells. 2022 Apr 7;11(8):1258. doi: 10.3390/cells11081258.
Human induced pluripotent stem cells (hiPSCs) are a promising cell source for elucidating disease pathology and therapy. The mass supply of hiPSC-derived cells is technically feasible. Carriers that can contain a large number of hiPSC-derived cells and evaluate their functions in vivo-like environments will become increasingly important for understanding disease pathogenesis or treating end-stage organ failure. hiPSC-derived hepatocyte-like cells (hiPSC-HLCs; 5 × 10) were seeded into decellularized organ-derived scaffolds under circumfusion culture. The scaffolds were implanted into immunodeficient microminiature pigs to examine their applicability in vivo. The seeded hiPSC-HLCs demonstrated increased albumin secretion and up-regulated cytochrome P450 activities compared with those in standard two-dimensional culture conditions. Moreover, they showed long-term survival accompanied by neovascularization in vivo. The decellularized organ-derived scaffold is a promising carrier for hiPSC-derived cells for ex vivo and in vivo use and is an essential platform for regenerative medicine and research.
人诱导多能干细胞（hiPSCs）是阐明疾病发病机制和治疗方法的有前途的细胞来源。大量供应 hiPSC 衍生细胞在技术上是可行的。能够容纳大量 hiPSC 衍生细胞并在类似于体内的环境中评估其功能的载体对于理解疾病发病机制或治疗终末期器官衰竭将变得越来越重要。将 5×10 的 hiPSC 衍生肝细胞样细胞（hiPSC-HLC）接种到细胞脱落后的器官衍生支架中进行灌注培养。将支架植入免疫缺陷型微型猪体内，以检查其在体内的适用性。与标准二维培养条件相比，接种的 hiPSC-HLC 表现出增加的白蛋白分泌和上调的细胞色素 P450 活性。此外，它们在体内表现出长期存活并伴有新血管生成。细胞脱落后的器官衍生支架是用于体外和体内使用的 hiPSC 衍生细胞的有前途的载体，是再生医学和研究的重要平台。
