Siebels Bente, Moritz Manuela, Hübler Diana, Gocke Antonia, Riedner Maria, Voß Hannah, Schlüter Hartmut
Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf, Martininstr. 52, Hamburg, 20246, Germany.
Eppendorf SE, Barkhausenweg 1, Hamburg, 22339, Germany.
Chemistry. 2024 Dec 5;30(68):e202403000. doi: 10.1002/chem.202403000. Epub 2024 Oct 16.
Analytes, from sample preparation, until entering an analytical instrument, are prone to adsorb to surfaces, driven by the chemical properties of the surface and the liquids they are dissolved in. This problem can be addressed with internal standards when a single or few known analytes are quantified that are usually not available in omics. However, minimal to no loss of analytes is the aim. Here, we present a novel assay for qualifying and quantifying interactions responsible for adsorption of molecules to surfaces (APS) by using LC-MS/MS-based differential quantitative analysis. To reflect a broad range of chemical interactions with surfaces, a reference mixture of thousands of tryptic peptides, with known compositions was selected, representing a variety of different chemical characteristics. The assay was tested by investigating the adsorption properties of several different vials with different surface chemistries. A significant number of hydrophobic peptides adsorbed to conventional polypropylene vials. In contrast, only few peptides adsorbed to polypropylene vials, assigned as low-protein-binding. The highest number of peptides adsorbed to glass vials driven by electrostatic interactions. In summary, the new assay is suitable to characterize adsorption properties of different surfaces and to approximate the loss of analytes during sample preparation.
从样品制备到进入分析仪器的分析物，由于表面及其所溶解液体的化学性质，容易吸附到表面。当对单种或少数几种通常在组学中不存在的已知分析物进行定量时，这个问题可以通过内标来解决。然而，目标是使分析物的损失最小化甚至没有损失。在这里，我们提出了一种新的分析方法，通过基于液相色谱-串联质谱的差异定量分析来鉴定和定量导致分子吸附到表面（APS）的相互作用。为了反映与表面的广泛化学相互作用，选择了一种已知组成的数千种胰蛋白酶肽的参考混合物，代表了各种不同的化学特性。通过研究几种具有不同表面化学性质的不同小瓶的吸附特性来测试该分析方法。大量疏水肽吸附到传统聚丙烯小瓶上。相比之下，只有少数肽吸附到被指定为低蛋白结合的聚丙烯小瓶上。由于静电相互作用，吸附到玻璃小瓶上的肽数量最多。总之，这种新的分析方法适用于表征不同表面的吸附特性，并估算样品制备过程中分析物的损失。
