Mohamed Abobakr A, Omar Mahmoud A, Alzahrani Eman, Abdel-Lateef Mohamed A, Mostafa Islam M
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, Fayoum, 63514, Egypt.
Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al- Madinah Al-Munawwarah, 41477, Saudi Arabia.
J Fluoresc. 2024 Aug 27. doi: 10.1007/s10895-024-03882-8.
A newly green method for the sensitive quantification of cloperastine, a cough suppressant, in spiked human plasma and its pharmaceutical formulation was designed for the first time. The established method depends on the enhancement of the weak fluorescence of cloperastine using 50 mM sulfuric acid to impair the photoinduced electron transfer produced from the nitrogen atom of piperidine moiety in cloperastine. This full protonation in an acid medium leads to an enhancement in the fluorescence of cloperastine, permitting its linear determination from 0.2 to 5.0 µg/mL with LOD and LOQ of 0.04 and 0.13 µg/mL, respectively. Moreover, the studied drug was estimated in its pharmaceutical market formulations as well as spiked human plasma. Furthermore, the greenness of the described method was evaluated.
首次设计了一种新型绿色方法，用于灵敏定量测定加标人血浆及其药物制剂中的镇咳药氯哌斯汀。所建立的方法依赖于使用50 mM硫酸增强氯哌斯汀的弱荧光，以削弱氯哌斯汀中哌啶部分氮原子产生的光诱导电子转移。在酸性介质中的这种完全质子化导致氯哌斯汀荧光增强，从而可以在0.2至5.0 µg/mL范围内对其进行线性测定，检测限和定量限分别为0.04和0.13 µg/mL。此外，还对所研究药物在其市售制剂以及加标人血浆中的含量进行了测定。此外，还评估了所描述方法的绿色度。
