Research Center for Natural Medicine and Chemical Metrology, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730101, China.
Anal Methods. 2024 Sep 19;16(36):6201-6209. doi: 10.1039/d4ay01034e.
As an important signaling molecule, carbon monoxide (CO) plays an important role in plant growth and development including affecting stomatal movement, stress response and root development. Thus, it is necessary to develop fluorescent probes that can be used to detect CO in live plant tissues and further enable a deep-understanding of its biological function, mechanism and metabolism. In this paper, a novel and sensitive fluorescent probe based on Cu modulated polydihydroxyphenylalanine nanoparticles (PDOAs) has been developed for the detection of CO. The fluorescence of PDOAs can be effectively quenched by Cu through the multi-coordination interaction. In the presence of CO, Cu can be effectively reduced to Cu, which resulted in the release of free PDOAs and the Cu-quenched bright green fluorescence was restored obviously. Through this ingenious strategy, the abiotic CO can be accurately detected and identified with high selectivity, rapid response time within 5 min and an ultralow detection limit of 72.4 nM. Due to the admirable biocompatibility, the nano-material based probe has been successfully applied for imaging CO in the root tip and leave tissues of lettuce. To the best of our knowledge, this is the first example of a fluorescent probe-based methodology for the sensitive tracking of CO in plant tissues.
一氧化碳（CO）作为一种重要的信号分子，在植物的生长发育过程中发挥着重要作用，包括影响气孔运动、应激反应和根发育。因此，有必要开发荧光探针来检测活植物组织中的 CO，从而进一步深入了解其生物学功能、机制和代谢。本文报道了一种基于 Cu 调控聚二羟基苯丙氨酸纳米粒子（PDOAs）的新型、灵敏的荧光探针，用于检测 CO。PDOAs 的荧光可以通过多配位相互作用被 Cu 有效地猝灭。在 CO 的存在下，Cu 可以被有效还原为 Cu，导致游离 PDOAs 的释放，Cu 猝灭的亮绿色荧光明显恢复。通过这种巧妙的策略，可以实现对非生物 CO 的准确检测和识别，具有高选择性、5 分钟内快速响应时间和超低检测限 72.4 nM。由于具有令人钦佩的生物相容性，该纳米材料基探针已成功应用于生菜根尖和叶片组织中 CO 的成像。据我们所知，这是首例基于荧光探针的方法用于植物组织中 CO 的灵敏追踪。
