1Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA), Avenue Ennasr, P.O. Box 415 Rabat Principal, 10090 Rabat, Morocco.
2Faculty of Sciences and Techniques, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, Hassan First University of Settat, P.O. Box 577, 26000 Settat, Morocco.
Acta Vet Hung. 2024 Aug 27;72(3):169-177. doi: 10.1556/004.2024.01074. Print 2024 Sep 12.
This interventional clinical trial aimed to assess the potential impact of Spirulina platensis supplementation on pregnant and lactating ewes living in a Moroccan endemic fluorosis area. Forty-eight ewes were divided into four equal groups: Groups I and II served as controls belonging respectively to fluorosis-free and endemic fluorosis areas, Groups III and IV received respectively 250 and 500 mgkg-1 BW/day of S. platensis, during late pregnancy and early lactation. The results revealed that ewes reared in fluorosis-free areas exhibited significantly lower plasma fluoride and significantly higher haemoglobin levels compared to endemic fluorosis areas (P < 0.0001). However, supplementation with 500 mgkg-1 BWday-1 of S. platensis significantly improved these two parameters compared to Group II (P < 0.0001). Ewes in the endemic area also displayed increased oxidative stress (P < 0.05), characterized by decreased ascorbic acid levels and catalase activity, as well as elevated levels of reduced glutathione and malondialdehyde. Supplementation with 500 mgkg-1 BW*day-1 of S. platensis enhanced the antioxidant status (P < 0.05) by increasing ascorbic acid levels and catalase activity and decreasing levels of reduced glutathione and malondialdehyde. Moreover, this dose yielded similar average daily gains compared to lambs of ewes living in fluorosis-free area. In conclusion, S. platensis may serve as a promising solution for addressing endemic fluorosis in pregnant and lactating ewes.
这项干预性临床试验旨在评估螺旋藻补充剂对生活在摩洛哥地方性氟中毒地区的妊娠和哺乳期母羊的潜在影响。将 48 只母羊分为四组，每组 12 只：第 I 组和第 II 组为对照组，分别来自无氟中毒区和地方性氟中毒区；第 III 组和第 IV 组在妊娠后期和哺乳期分别接受 250 和 500 mgkg-1 BW/天的螺旋藻补充剂。结果表明，无氟中毒区饲养的母羊的血浆氟含量明显低于地方性氟中毒区（P < 0.0001），而血红蛋白水平明显高于后者（P < 0.0001）。然而，与第 II 组相比，每天补充 500 mgkg-1 BW 的螺旋藻可显著改善这两个参数（P < 0.0001）。地方性氟中毒区的母羊还表现出氧化应激增加（P < 0.05），其特征是抗坏血酸水平和过氧化氢酶活性降低，以及还原型谷胱甘肽和丙二醛水平升高。补充 500 mg*kg-1 BW 的螺旋藻可通过增加抗坏血酸水平和过氧化氢酶活性以及降低还原型谷胱甘肽和丙二醛水平来增强抗氧化状态（P < 0.05）。此外，该剂量的螺旋藻对羔羊的平均日增重与无氟中毒区的羔羊相似。总之，螺旋藻可能是解决妊娠和哺乳期母羊地方性氟中毒的一种有前途的方法。
