Gut promotes protective immunity to foodborne infection.

UNLABELLED

The gut microbiota, a pivotal component of the intestinal mucosal barrier, is critical for host resistance to enteric pathogen infection. Here, we report a novel function of the potentially probiotic strain LG1 ( strain LG1) in maintaining intestinal mucosal barrier integrity and protecting against foodborne () infection. was isolated from the intestinal contents of Chinese Mongolian sheep (MS) and exhibited potential probiotic properties. In a enterocolitis model, pretreated mice were less susceptible to infection compared with Phosphate buffered solution (PBS)pretreated mice, which manifested as higher survival rates, lower pathogen loads, less weight loss, mild clinical symptoms and intestinal damage, and minor inflammation. Further mechanistic analysis showed that could ameliorate the disruption of intestinal permeability and maintain the integrity of the intestinal mucosal barrier by promoting the expression of tight junction proteins and mucoproteins. Moreover, was also able to facilitate antimicrobial peptide expression and ameliorate dysbiosis of the gut microbiota caused by . Together, these findings highlight the prospect of immunomodulatory potentially probiotic and might offer valuable strategies for prophylaxis and/or treatment of pathogenic mucosal infection.

IMPORTANCE

necrotic enteritis leads to losses of about US $2 billion to the poultry industry worldwide every year. Worse, US Centers for Disease Control and Prevention (CDC) has estimated that causes nearly 1 million foodborne illnesses in the United States annually. Nowadays, the treatment recommendation is a combination of a broad-spectrum synergistic penicillin with clindamycin or a carbapenem, despite growing scientific concern over antibiotic resistance. The global understanding of the gut microbiome for infection may provide important insights into the intervention. originated from Mongolian sheep intestine, exhibited potentially probiotic properties, and was able to limit enterocolitis and pathogenic colonization. Importantly, we found that limits invasion via improving intestinal mucosal barrier function. Also, alleviates -induced gut microbiota dysbiosis. It allowed us to convince that utilization of probiotics to promote protective immunity against pathogens infection is of pivotal importance.