Lac16对IPEC-J2细胞感染相关损伤的保护作用。

Protective Effects of Lac16 on Infection-Associated Injury in IPEC-J2 Cells.

作者信息

Zhou Yuanhao, Wang Baikui, Wang Qi, Tang Li, Zou Peng, Zeng Zihan, Zhang Huihua, Gong Li, Li Weifen

机构信息

Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science (Eastern of China) of the Ministry of Agriculture, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

Department of Animal Sciences, School of Life Science and Engineering, Foshan University, Foshan 528225, China.

出版信息

Int J Mol Sci. 2021 Nov 17;22(22):12388. doi: 10.3390/ijms222212388.

() causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of () Lac16 on infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that Lac16 significantly inhibited the growth of , which was accompanied by a decrease in pH levels. In addition, Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of to IPEC-J2 cells, and attenuated -induced cellular cytotoxicity and intestinal barrier damage. Furthermore, Lac16 significantly suppressed -induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by infection. Collectively, probiotic Lac16 exerts protective effects against infection-associated injury in IPEC-J2 cells.

（某菌）通过过度生长和分泌多种毒素导致肠道损伤，在包括猪、鸡和羊在内的动物中引发腹泻和坏死性肠炎。本研究旨在探讨（某益生菌）Lac16对猪肠道上皮细胞系（IPEC-J2）中与（某菌）感染相关损伤的保护作用。结果表明，Lac16显著抑制了（某菌）的生长，同时pH值降低。此外，Lac16显著提高了IPEC-J2细胞中宿主防御肽（HDPs）的mRNA表达水平，降低了（某菌）对IPEC-J2细胞的粘附，并减轻了（某菌）诱导的细胞毒性和肠道屏障损伤。此外，Lac16显著抑制了（某菌）诱导的IPEC-J2细胞中促炎细胞因子和模式识别受体（PRRs）的基因表达。此外，Lac16预孵育有效抑制了（某菌）感染引起的p65磷酸化。总体而言，益生菌Lac16对IPEC-J2细胞中与（某菌）感染相关的损伤具有保护作用。