State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
J Agric Food Chem. 2022 Nov 23;70(46):14665-14678. doi: 10.1021/acs.jafc.2c04602. Epub 2022 Nov 15.
This study focused on the effects of CCFM1078 on the intestinal barrier and systemic inflammation of collagen-induced arthritis (CIA) rats. Female rats were divided into three groups with daily intragastric administration of either saline (control group and model group) or CCFM1078 (CCFM1078 group, 3 × 10cfu/rat per day) for 5 weeks. In the Model and CCFM1078 groups, arthritis was induced by subcutaneous collagen injection. We found that CCFM1078 can repair the intestinal barrier, reduce LPS translocation, regulate gut microbiota composition, and increase short-chain fatty acids in the intestine. Then, it can reduce pro-inflammatory cytokines release, adjust immune dysfunction, and inhibit TLR4-MyD88-dependent pathways and downstream inflammatory pathways to alleviate joint inflammation in CIA rats. These findings suggest that CCFM1078 may alleviate joint inflammation by adjusting the profile of gut microbiota and enhancing the intestinal barrier.
本研究聚焦于 CCFM1078 对胶原诱导关节炎（CIA）大鼠肠道屏障和全身炎症的影响。雌性大鼠分为三组，每天分别灌胃生理盐水（对照组和模型组）或 CCFM1078（CCFM1078 组，每天 3×10cfu/大鼠），持续 5 周。在模型组和 CCFM1078 组中，关节炎通过皮下胶原注射诱导。我们发现 CCFM1078 可以修复肠道屏障，减少 LPS 易位，调节肠道微生物组成，并增加肠道内短链脂肪酸。然后，它可以减少促炎细胞因子的释放，调节免疫功能障碍，并抑制 TLR4-MyD88 依赖性途径和下游炎症途径，从而减轻 CIA 大鼠的关节炎症。这些发现表明，CCFM1078 可能通过调节肠道微生物群的特征和增强肠道屏障来缓解关节炎症。
