因子VIII相关抗原增强发光酶免疫测定法
Enhanced luminescence enzyme immunoassay for factor VIII related antigen.
作者信息
Wang H X, George J, Thorpe G H, Stott R A, Kricka L J, Whitehead T P
出版信息
J Clin Pathol. 1985 Mar;38(3):317-9. doi: 10.1136/jcp.38.3.317.
A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).
已开发出一种用于检测血浆因子 VIII 相关抗原的夹心酶免疫测定法，该方法利用对碘苯酚增强化学发光反应来检测辣根过氧化物酶标记物。该测定法包括与样品和结合物进行 15 分钟的孵育，检测限为 0.12 mU。它显示出良好的批内精密度（变异系数 = 2.95 - 5.8%），并且对 57 个样本系列的检测结果与免疫电泳获得的结果一致（相关系数 = 0.97）。
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