Woods D E, Bryan L E
J Infect Dis. 1985 Apr;151(4):581-8. doi: 10.1093/infdis/151.4.581.
Most patients with cystic fibrosis become colonized and infected with mucoid Pseudomonas aeruginosa. The major component of the mucoid material has been identified as the polysaccharide alginic acid. The present work was undertaken to determine whether antibody to alginate is protective in a model of chronic lung infection with P. aeruginosa in rats. Bacterial clearance was associated with a rise in titers of antibody to alginate. In a number of animals a rise in antibody titers was not seen, and in fact a decrease was noted at 30 days compared with 10 days. This observation suggested the possibility of immune complex formation due to antigen excess. Evidence for immune complex deposition in tissues was obtained by immunofluorescence studies. Thus antibody to alginate may offer strain-dependent protection against chronic lung infection with P. aeruginosa in rats; however, immune complex formation should be considered as a possible consequence of immunization with alginate.
大多数囊性纤维化患者会被黏液型铜绿假单胞菌定植并感染。黏液物质的主要成分已被鉴定为多糖藻酸盐。目前的研究旨在确定抗藻酸盐抗体在大鼠铜绿假单胞菌慢性肺部感染模型中是否具有保护作用。细菌清除与抗藻酸盐抗体滴度升高有关。在一些动物中未观察到抗体滴度升高，事实上，与10天时相比，30天时抗体滴度出现了下降。这一观察结果提示了由于抗原过量而形成免疫复合物的可能性。通过免疫荧光研究获得了免疫复合物在组织中沉积的证据。因此，抗藻酸盐抗体可能对大鼠铜绿假单胞菌慢性肺部感染提供菌株依赖性保护；然而，免疫复合物的形成应被视为用藻酸盐免疫的一个可能后果。
