Young E J, Borchert M, Kretzer F L, Musher D M
J Infect Dis. 1985 Apr;151(4):682-90. doi: 10.1093/infdis/151.4.682.
Although cellular immunity involving activated macrophages is important in resistance to Brucella, serum factors and polymorphonuclear leukocytes (PMNLs) play some role in the initial response to infection. The interaction between human PMNLs and virulent and attenuated strains of Brucella abortus and Brucella melitensis was studied by in vitro techniques. Virulent and attenuated strains of both species were rapidly phagocytosed after opsonization with normal human serum (NHS); nonopsonized bacteria were not phagocytosed. In contrast, NHS devoid of detectable antibodies was bactericidal for strains of B. abortus but not of B. melitensis. In addition, intracellular killing of ingested bacteria was shown for virulent B. abortus but not for B. melitensis. Ultrastructural studies revealed morphological alterations in about one-half of phagocytosed B. abortus and B. melitensis after incubation for 10 min; thereafter, nearly 100% of B. abortus showed some degree of degeneration, whereas B. melitensis remained intact during 120 min of observation.
虽然涉及活化巨噬细胞的细胞免疫在抵抗布鲁氏菌方面很重要，但血清因子和多形核白细胞（PMNLs）在感染的初始反应中也发挥了一定作用。通过体外技术研究了人PMNLs与流产布鲁氏菌和羊布鲁氏菌的强毒株及减毒株之间的相互作用。两种菌的强毒株和减毒株在用正常人血清（NHS）调理后都能迅速被吞噬；未调理的细菌则不被吞噬。相比之下，缺乏可检测抗体的NHS对流产布鲁氏菌菌株有杀菌作用，但对羊布鲁氏菌则没有。此外，已显示摄入的细菌在细胞内可被杀死，这在强毒流产布鲁氏菌中是如此，但在羊布鲁氏菌中则不然。超微结构研究显示，吞噬的流产布鲁氏菌和羊布鲁氏菌在孵育10分钟后约有一半出现形态改变；此后，近100%的流产布鲁氏菌呈现出一定程度的退化，而羊布鲁氏菌在120分钟的观察期内保持完整。
