Plant Protection Department, Shiraz University, Shiraz, Iran.
Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia (MBG-CSIC), Apartado 28, 36080, Pontevedra, Spain.
Sci Rep. 2024 Aug 27;14(1):19865. doi: 10.1038/s41598-024-68345-3.
To investigate the effects of temperature on Brassica napus (canola) resistance to Leptosphaeria maculans (LM), the causal agent of blackleg disease, metabolic profiles of LM infected resistant (R) and susceptible (S) canola cultivars at 21 °C and 28 °C were analyzed. Metabolites were detected in cotyledons of R and S plants at 48- and 120-h post-inoculation with LM using UPLC-QTOF/MS. The mock-inoculated plants were used as controls. Some of the resistance-related specific pathways, including lipid metabolism, amino acid metabolism, carbohydrate metabolism, and aminoacyl-tRNA biosynthesis, were down-regulated in S plants but up-regulated in R plants at 21 °C. However, some of these pathways were down-regulated in R plants at 28 °C. Amino acid metabolism, lipid metabolism, alkaloid biosynthesis, phenylpropanoid biosynthesis, and flavonoid biosynthesis were the pathways linked to combined heat and pathogen stresses. By using network analysis and enrichment analysis, these pathways were identified as important. The pathways of carotenoid biosynthesis, pyrimidine metabolism, and lysine biosynthesis were identified as unique mechanisms related to heat stress and may be associated with the breakdown of resistance against the pathogen. The increased susceptibility of R plants at 28 °C resulted in the down-regulation of signal transduction pathway components and compromised signaling, particularly during the later stages of infection. Deactivating LM-specific signaling networks in R plants under heat stress may result in compatible responses and deduction in signaling metabolites, highlighting global warming challenges in crop disease control.
为了研究温度对油菜（甘蓝型油菜）抵抗黑胫病菌（引起黑胫病的病原体）的影响，在 21°C 和 28°C 下分析了抗（R）和感（S）油菜品种感染 LM 后的代谢谱。使用 UPLC-QTOF/MS 在 LM 接种后 48 小时和 120 小时检测了 R 和 S 植株子叶中的代谢物。模拟接种的植株用作对照。在 21°C 下，一些与抗性相关的特定途径，包括脂质代谢、氨基酸代谢、碳水化合物代谢和氨酰-tRNA 生物合成，在 S 植株中下调，但在 R 植株中上调。然而，在 28°C 下，这些途径在 R 植株中下调。氨基酸代谢、脂质代谢、生物碱生物合成、苯丙烷生物合成和类黄酮生物合成是与热和病原体胁迫相结合的途径。通过使用网络分析和富集分析，这些途径被确定为重要途径。类胡萝卜素生物合成、嘧啶代谢和赖氨酸生物合成途径被确定为与热应激相关的独特机制，可能与对病原体抗性的破坏有关。在 28°C 下，R 植株的易感性增加导致信号转导途径成分下调和信号受损，特别是在感染后期。在热应激下，使 R 植株中的 LM 特异性信号网络失活可能导致相容反应和信号代谢物减少，突出了全球变暖对作物疾病控制的挑战。
