Department of Spinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Department of Orthopedic Surgery, Fujian Medical University Union Hospital, Fuzhou, China.
Methods Mol Biol. 2025;2854:83-91. doi: 10.1007/978-1-0716-4108-8_10.
Transcriptomics is an extremely important area of molecular biology and is a powerful tool for studying all RNA molecules in an organism. Conventional transcriptomic technologies include microarrays and RNA sequencing, and the rapid development of single-cell sequencing and spatial transcriptomics in recent years has provided an enormous scope for research in this field. This chapter describes the application, significance, and experimental procedures of a variety of transcriptomic technologies in antiviral natural immunity.
转录组学是分子生物学中一个极其重要的领域，是研究生物体内所有 RNA 分子的有力工具。传统的转录组学技术包括微阵列和 RNA 测序，近年来单细胞测序和空间转录组学的快速发展为该领域的研究提供了广阔的空间。本章描述了各种转录组学技术在抗病毒天然免疫中的应用、意义和实验程序。
