酵母双杂交技术检测抗病毒固有免疫。

Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity.

机构信息

Department of Biological Engineering, Wuhan Polytechnic University, Wuhan, China.

State Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, China.

出版信息

Methods Mol Biol. 2025;2854:213-220. doi: 10.1007/978-1-0716-4108-8_21.

DOI:10.1007/978-1-0716-4108-8_21

PMID:39192132

Abstract

Yeast two-hybrid (YTH) technology is a powerful tool for studying protein interactions and has been widely used in various fields of molecular biology, including the study of antiviral innate immunity. This chapter presents detailed information and experimental procedures for identifying virus-host protein interactions involved in immune regulation using yeast two-hybrid technology.

摘要

酵母双杂交（YTH）技术是研究蛋白质相互作用的强大工具，已广泛应用于分子生物学的各个领域，包括抗病毒先天免疫的研究。本章介绍了使用酵母双杂交技术鉴定参与免疫调节的病毒-宿主蛋白相互作用的详细信息和实验步骤。