Kunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Institute of Pediatrics, Yunnan Province Clinical Research Center for Children's Health and Disease, Kunming Children's Hospital, Kunming, Yunnan, China.
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2025;2854:253-264. doi: 10.1007/978-1-0716-4108-8_24.
Innate immunity is an important defense barrier for the human body. After viral pathogen-associated molecular patterns (PAMPs) are detected by host-pathogen recognition receptors (PRRs), the associated signaling pathways trigger the activation of the interferon (IFN) regulatory factor (IRF) family members and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). However, any gene defects among the signaling adaptors will compromise innate immune efficiency. Therefore, investigating genetic defects in the antiviral innate immune signaling pathway is important. We summarize the commonly used research methods related to antiviral immune gene defects and outline the relevant research protocols, which will help investigators study antiviral innate immunity.
先天免疫是人体的重要防御屏障。病毒病原体相关分子模式（PAMPs）被宿主-病原体识别受体（PRRs）检测到后，相关信号通路会触发干扰素（IFN）调节因子（IRF）家族成员和核因子κB 轻链增强子的激活B 细胞（NF-κB）。然而，信号适配器中的任何基因缺陷都会影响先天免疫效率。因此，研究抗病毒先天免疫信号通路中的遗传缺陷非常重要。我们总结了与抗病毒免疫基因缺陷相关的常用研究方法，并概述了相关的研究方案，这将有助于研究人员研究抗病毒先天免疫。
