蛋白激酶 R（PKR）作为抗病毒先天免疫中的新型 dsRNA 传感器。
Protein Kinase R (PKR) as a Novel dsRNA Sensor in Antiviral Innate Immunity.
机构信息
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA.
Laboratory of Viral Diseases, NIAID NIH, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2025;2854:265-282. doi: 10.1007/978-1-0716-4108-8_25.
Protein kinase R (PKR), a key double-stranded RNA (dsRNA)-activated sensor, is pivotal for cellular responses to diverse stimuli. This protocol delineates a comprehensive methodological framework employing single luciferase assays, yeast assays, immunoblot assays, and quantitative PCR (qPCR) to discern and validate PKR activities and their downstream impacts on NF-κB-activating signaling pathways. These methodologies furnish a systematic approach to unraveling the role of PKR as a dsRNA sensor and effector in antiviral innate immunity, enabling in-depth analyses of dsRNA sensor activities.
蛋白激酶 R（PKR）是一种关键的双链 RNA（dsRNA）激活传感器，对于细胞对各种刺激的反应至关重要。本方案描述了一种综合的方法学框架，包括使用单个荧光素酶测定、酵母测定、免疫印迹测定和定量 PCR（qPCR）来辨别和验证 PKR 活性及其对 NF-κB 激活信号通路的下游影响。这些方法提供了一种系统的方法来揭示 PKR 作为 dsRNA 传感器和抗病毒先天免疫效应子的作用，能够深入分析 dsRNA 传感器的活性。
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