Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People's Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People's Hospital, University of Traditional Chinese Medicine), No.33, Mashi Street, Wenjiang District, Chengdu City, Sichuan Province, 611130, China.
Chengdu Railway Health School, Chengdu City, Sichuan Province, 611741, China.
Hereditas. 2024 Aug 27;161(1):26. doi: 10.1186/s41065-024-00328-w.
Non-small cell lung cancer (NSCLC) patients undergoing chemotherapy and immunotherapy experience disturbances in the gut microbiota. This study intends to find out the correlation between gut microbiota and clinical indices before and after radiotherapy for NSCLC.
Ten patients with primary NSCLC were screened, and plasma and fecal samples were collected before and after radiotherapy, respectively. Inflammatory indices in plasma were detected. Genomic DNA was extracted from fecal specimens and sequenced on on Illumina HiSeq2000 sequencing platform. Thee sequenced data were subjected to Metagenome assembly, gene prediction, species annotation, and gene function analysis to study and analyze gut microbiota and metabolic functions. The correlation between the diversity of gut microbiota and the clinical indicators of NSCLC patients was evaluated, and the changes of gut microbiota before and after radiotherapy were observed.
The diversity of gut microbiota in NSCLC patients did not correlate with smoking, pathology, and inflammatory markers. The abundance of phylum (p)_Bacteroidetes increased; p_Firmicutes and p_Bacteroidetes accounted for the highest proportion in NSCLC patients, and the abundance of both was dominantly exchanged after radiotherapy. There was a decrease in genus (g)_Bifidobacterium after radiotherapy in NSCLC patients. There was no significant correlation between the diversity of gut microbiota after radiotherapy and radiotherapy sensitivity, and the structural composition and abundance of gut microbiota remained stable.
The diversity of gut microbiota is altered after radiotherapy in NSCLC patients, showing an increase in harmful bacteria and a decrease in beneficial bacteria.
接受化疗和免疫治疗的非小细胞肺癌（NSCLC）患者肠道微生物群会发生紊乱。本研究旨在探讨 NSCLC 患者放疗前后肠道微生物群与临床指标的相关性。
筛选 10 例初治 NSCLC 患者，分别采集放疗前后的血浆和粪便标本，检测血浆中的炎症指标。从粪便标本中提取基因组 DNA，在 Illumina HiSeq2000 测序平台上进行测序。对测序数据进行宏基因组组装、基因预测、物种注释和基因功能分析，研究和分析肠道微生物群和代谢功能。评估肠道微生物群多样性与 NSCLC 患者临床指标的相关性，并观察放疗前后肠道微生物群的变化。
NSCLC 患者肠道微生物群的多样性与吸烟、病理和炎症标志物均无相关性。门（p）_拟杆菌门的丰度增加；p_厚壁菌门和 p_拟杆菌门在 NSCLC 患者中占比最高，放疗后两者的丰度均发生明显交换。g_双歧杆菌属在放疗后 NSCLC 患者中的丰度下降。放疗后肠道微生物群的多样性与放疗敏感性之间无显著相关性，肠道微生物群的结构组成和丰度保持稳定。
NSCLC 患者放疗后肠道微生物群的多样性发生改变，表现为有害菌增多、有益菌减少。
