Abdelmajed Mahmoud A, El-Din Khalid M Badr, Attia Tamer Z, Omar Mahmoud A
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Deraya University, New Minia, Egypt.
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.
BMC Chem. 2024 Aug 27;18(1):158. doi: 10.1186/s13065-024-01261-9.
Due to the lack of other treatment options, a rebirth of polymyxins is urgently required. Colistin (also called polymyxin E) and polymyxin B are the only two examples of this antibiotic class that were effectively employed in such critical situations. In the present work, both of the two studied medications were quantified via a simple, green, and non-extracting spectrophotometric approach based on the formation of ion-pair complexes with Erythrosine B. Without using any organic solvents, the pink color of the created complexes was detected at wavelength = 558 nm. To achieve the highest intensity of absorbance, optimum conditions were established by the screening of many experimental factors such as pH, buffer volume, the volume of Erythrosine B, and the time consumed to undergo the reaction. For Colistin and Polymyxin B respectively, Beer-Lambert's law was observed at the concentration ranges of 1-6, 1-9 µg mL. The technique was approved and validated following ICH recommendations. Lastly, the suggested approach has been successfully implemented to quantify the cited medications colorimetrically, for the first time, in their parenteral dosage forms with excellent recoveries. Also, Content uniformity testing was implemented.
由于缺乏其他治疗选择，迫切需要让多粘菌素重获新生。黏菌素（也称为多粘菌素E）和多粘菌素B是这类抗生素中仅有的两种在如此危急情况下得到有效应用的药物。在本研究中，基于与赤藓红B形成离子对络合物，通过一种简单、绿色且无需萃取的分光光度法对这两种研究药物进行了定量。无需使用任何有机溶剂，在波长 = 558 nm处检测到所形成络合物的粉红色。通过筛选许多实验因素，如pH值、缓冲液体积、赤藓红B的体积以及反应所需时间，确定了达到最高吸光度强度的最佳条件。对于黏菌素和多粘菌素B，分别在1 - 6、1 - 9 μg/mL的浓度范围内符合比尔-朗伯定律。该技术按照国际人用药品注册技术协调会（ICH）的建议进行了验证。最后，所建议的方法首次成功用于比色法定量上述药物的注射剂剂型，回收率良好。此外，还进行了含量均匀度测试。
