Zabell Adam P R, Lytle Fred E, Julian Randall K
Indigo BioAutomation, 7820 Innovation Blvd, Suite 250, Indianapolis, IN 46278, United States.
Clin Mass Spectrom. 2017 Jan 3;2:25-33. doi: 10.1016/j.clinms.2016.12.003. eCollection 2016 Dec.
Instrument calibration, required for any accurate quantitative calculation, is a trivial process when performed correctly, but is also full of easily overlooked stumbling blocks. To minimize the risk of error associated with improper calibrations, national and international guidance dictates a minimum number of calibrators and the threshold at which a measurement becomes an outlier. Evidence from industry practice, which conflicts with regulatory guidance, suggests that most groups are focused on remapping their detector with each run. We present a explanation for the calibrator minimum and provide recommendations for curve building, which include improved outlier detection for high-volume mass spectrometry laboratories.
仪器校准是任何精确的定量计算所必需的，若操作正确，这是一个简单的过程，但也充满了容易被忽视的障碍。为了将与校准不当相关的误差风险降至最低，国家和国际指南规定了校准物的最少数量以及测量成为异常值的阈值。来自行业实践的证据与监管指南相冲突，表明大多数团队每次运行时都专注于重新映射其检测器。我们对校准物的最少数量给出了解释，并为曲线构建提供了建议，其中包括为高容量质谱实验室改进异常值检测。
