Infectious diseases research center, Golestan University of Medical Sciences, Gorgan, Iran.
Department of Basic Sciences, Khoy University of Medical Sciences, Khoy, Iran.
Arch Razi Inst. 2024 Feb 1;79(1):111-119. doi: 10.32592/ARI.2024.79.1.111. eCollection 2024 Feb.
Hepatitis B virus (HBV) X protein (HBx) plays a key role in hepatocellular carcinoma (HCC). HBx may alter the expression of multiple microRNAs (miRs), which are important in hepatocarcinogenesis. This study aimed to investigate the importance of HBx protein in the expression of miR-21, miR-22, miR-122, miR-132, and miR-222. A recombinant vector expressing HBx was developed. The Huh-7 cell line was transfected with the HBx-pcDNA3.1+ recombinant plasmid. A Real-Time Polymerase Chain Reaction was used to evaluate the expression of miR-21, miR-22, miR-122, miR-132, and miR-222 in the cell line. It was found that the expression of miR-21 and miR-222 was upregulated at all points of time after HBx transfection. The expression of miR-21 was 4.24-fold 72 h after transfection. The miR-22 had a 7.69-fold downregulation after 24 h, and the miR-122 had a significant downregulation after 48 h (10-fold). The miR-132 expression reached its lowest rate 12 h after HBx transfection (8.33-fold), and the miR-222 expression was upregulated in transfected cells but was not significantly different (1.18- to 2.45-fold). The significant downregulation of miR-22, miR-122, and miR-132 implicates their inhibitory roles in the progression of HBV-associated HCC. The expression of these microRNAs could be used as a prognostic marker for the progression of HBV-associated liver disease.
乙型肝炎病毒 (HBV) X 蛋白 (HBx) 在肝细胞癌 (HCC) 中发挥关键作用。HBx 可能改变多个 microRNAs (miRs) 的表达，miRs 在肝癌发生中非常重要。本研究旨在探讨 HBx 蛋白在 miR-21、miR-22、miR-122、miR-132 和 miR-222 表达中的重要性。构建表达 HBx 的重组载体。将 Huh-7 细胞系转染 HBx-pcDNA3.1+重组质粒。使用实时聚合酶链反应评估细胞系中 miR-21、miR-22、miR-122、miR-132 和 miR-222 的表达。结果发现，转染 HBx 后所有时间点 miR-21 和 miR-222 的表达均上调。转染后 72 h miR-21 表达上调 4.24 倍。miR-22 在 24 h 时下调 7.69 倍，miR-122 在 48 h 时显著下调（10 倍）。miR-132 的表达在 HBx 转染后 12 h 达到最低（8.33 倍），转染细胞中 miR-222 的表达上调，但无统计学差异（1.18-2.45 倍）。miR-22、miR-122 和 miR-132 的显著下调表明它们在 HBV 相关 HCC 的进展中具有抑制作用。这些 microRNAs 的表达可以作为 HBV 相关肝病进展的预后标志物。
