Deng Jia, Ye Yu-Jian, Chen Qiu-Ping, Zhang Yi-Jin, Liu Ji-Feng
Department of Dermatology, Hangzhou Third People's Hospital, Hangzhou, People's Republic of China.
Clin Cosmet Investig Dermatol. 2024 Aug 23;17:1887-1893. doi: 10.2147/CCID.S470020. eCollection 2024.
To assess the accuracy of HSV1and HSV2 antibody testing in identifying genital herpes infection.
A cohort of 299 patients previously diagnosed with recurrent genital herpes, confirmed via PCR, were tested using ELISA for HSV1 and HSV2 IgM and IgG antibodies. The study compared the accuracy of HSV1 and HSV2 antibody tests in diagnosing genital herpes.
Among 299 patients, 14 tested positives for HSV1 DNA. Of these, 9 had HSV1 IgG antibodies, but none had HSV2 IgG antibody. Among 278 patients with HSV2 DNA, 149 had HSV1 IgG, 9 had HSV2 IgG, and 97 had both. Seven patients had both HSV1 and HSV2 DNA; 3 had HSV1 IgG, 1 had HSV2 IgG, and 3 had both. The accuracy of HSV1 IgG for HSV1 infection was 64.2%, and for HSV1 and HSV2 co-infection, 85.7%. The accuracy of HSV2 IgG for HSV2 infection was 38.1%, and for HSV1 and HSV2 co-infection, 57.1%. The combined antibody positivity accuracy was 34.9%.
Genital herpes is primarily caused by HSV2 (92.98%). A smaller percentage is HSV1 (4.67%) or co-infection (2.34%). Despite relatively low diagnostic accuracy (34.9-85.7%) for antibody detection, combined antibody testing is necessary. Herpes DNA testing is recommended for accurate diagnosis. Absence of antibodies does not rule out genital herpes and clinical assessment is essential.
评估单纯疱疹病毒1型（HSV1）和单纯疱疹病毒2型（HSV2）抗体检测在诊断生殖器疱疹感染中的准确性。
对299例先前经聚合酶链反应（PCR）确诊为复发性生殖器疱疹的患者进行研究，采用酶联免疫吸附测定（ELISA）检测HSV1和HSV2 IgM及IgG抗体。本研究比较了HSV1和HSV2抗体检测在诊断生殖器疱疹方面的准确性。
299例患者中，14例HSV1 DNA检测呈阳性。其中，9例有HSV1 IgG抗体，但均无HSV2 IgG抗体。278例HSV2 DNA阳性患者中，149例有HSV1 IgG，9例有HSV2 IgG，97例两者均有。7例患者同时有HSV1和HSV2 DNA；3例有HSV1 IgG，1例有HSV2 IgG，3例两者均有。HSV1 IgG对HSV1感染的诊断准确性为64.2%，对HSV1和HSV2合并感染的诊断准确性为85.7%。HSV2 IgG对HSV2感染的诊断准确性为38.1%，对HSV1和HSV2合并感染的诊断准确性为57.1%。联合抗体阳性的诊断准确性为34.9%。
生殖器疱疹主要由HSV2引起（92.98%）。较小比例由HSV1引起（4.67%）或合并感染（2.34%）。尽管抗体检测的诊断准确性相对较低（34.9%-85.7%），但联合抗体检测是必要的。建议进行疱疹DNA检测以获得准确诊断。抗体阴性不能排除生殖器疱疹，临床评估至关重要。
